Cellular analysis of the mode of action of methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME) on tumor cells

  • Authors:
    • J Zhen
    • B Feuerstein
    • C Vidair
    • W Hyun
    • L Young
    • E Kirsten
    • E Kun
  • View Affiliations

  • Published online on: May 1, 1997     https://doi.org/10.3892/ijo.10.5.905
  • Pages: 905-910
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Abstract

The hormonally inactive methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME) at 1-4 mu M concentration induces morphologic changes in E-ras 20 and MDA-MB-231 and other human cancer cells such as multinucleation and enlargement and arrests the cell cycle in the M phase without affecting interphase. Time-lapse videomicroscopy allowed us to follow individual cells. Cells exposed to DIME divided in an abnormal manner, leading to 20% cell fusion and multinucleation. Chromosome painting demonstrated a large accumulation of chromosomes after 5 days of treatment with DIME, consistent with the failure of cells to divide normally. Chromosome breakage was not observed. On the other hand, highly abnormal tubulin-containing structures ensue upon exposure to DIME (1 mu M for 18 h) treatment, indicating an early biochemical action of DIME on the spindle assembly system.

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May 1997
Volume 10 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Zhen J, Feuerstein B, Vidair C, Hyun W, Young L, Kirsten E and Kun E: Cellular analysis of the mode of action of methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME) on tumor cells. Int J Oncol 10: 905-910, 1997
APA
Zhen, J., Feuerstein, B., Vidair, C., Hyun, W., Young, L., Kirsten, E., & Kun, E. (1997). Cellular analysis of the mode of action of methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME) on tumor cells. International Journal of Oncology, 10, 905-910. https://doi.org/10.3892/ijo.10.5.905
MLA
Zhen, J., Feuerstein, B., Vidair, C., Hyun, W., Young, L., Kirsten, E., Kun, E."Cellular analysis of the mode of action of methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME) on tumor cells". International Journal of Oncology 10.5 (1997): 905-910.
Chicago
Zhen, J., Feuerstein, B., Vidair, C., Hyun, W., Young, L., Kirsten, E., Kun, E."Cellular analysis of the mode of action of methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME) on tumor cells". International Journal of Oncology 10, no. 5 (1997): 905-910. https://doi.org/10.3892/ijo.10.5.905