Establishment and characterization of a new human gallbladder carcinoma cell line (OCUG-1) producing TA-4

  • Authors:
    • N Yamada
    • Y Chung
    • H Ohtani
    • T Ikeda
    • N Onoda
    • T Sawada
    • Y Nishiguchi
    • T Hasuma
    • M Sowa
  • View Affiliations

  • Published online on: June 1, 1997     https://doi.org/10.3892/ijo.10.6.1251
  • Pages: 1251-1255
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Abstract

A new human cell line (OCUG-1) was established from peritoneal effusion of a patient with malignant gallbladder carcinoma. OCUG-1 cells proliferated mainly in clusters of cells partially floating in a monolayered sheet. The population doubling time is 47.1 h. DNA analysis showed that OCUG-1 cells were aneuploid and had two G(0)/G(1) peaks. The number of chromosomes was distributed in a broad range from 52 to 139. Subcutaneous injections of the cells induced tumor formation in all nude mice. The reconstituted tumors were poorly differentiated adenocarcinoma. High levels of SLX, CA19-9, SPan-1 and TA-4 were found in the serum of the original patient, but OCUG-1 cells produced only TA-4. We speculate that OCUG-1 may be a transitional form from adenocarcinoma to squamous cell carcinoma. Since OCUG-1 produces a high level of TA-4, it will be useful for the study of the biological nature of this carcinoma and the relationship between the expression of TA-4 and squamatization.

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June 1997
Volume 10 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Yamada N, Chung Y, Ohtani H, Ikeda T, Onoda N, Sawada T, Nishiguchi Y, Hasuma T and Sowa M: Establishment and characterization of a new human gallbladder carcinoma cell line (OCUG-1) producing TA-4. Int J Oncol 10: 1251-1255, 1997
APA
Yamada, N., Chung, Y., Ohtani, H., Ikeda, T., Onoda, N., Sawada, T. ... Sowa, M. (1997). Establishment and characterization of a new human gallbladder carcinoma cell line (OCUG-1) producing TA-4. International Journal of Oncology, 10, 1251-1255. https://doi.org/10.3892/ijo.10.6.1251
MLA
Yamada, N., Chung, Y., Ohtani, H., Ikeda, T., Onoda, N., Sawada, T., Nishiguchi, Y., Hasuma, T., Sowa, M."Establishment and characterization of a new human gallbladder carcinoma cell line (OCUG-1) producing TA-4". International Journal of Oncology 10.6 (1997): 1251-1255.
Chicago
Yamada, N., Chung, Y., Ohtani, H., Ikeda, T., Onoda, N., Sawada, T., Nishiguchi, Y., Hasuma, T., Sowa, M."Establishment and characterization of a new human gallbladder carcinoma cell line (OCUG-1) producing TA-4". International Journal of Oncology 10, no. 6 (1997): 1251-1255. https://doi.org/10.3892/ijo.10.6.1251