Surface plasmon resonance for real-time detection of molecular interactions between chromomycin and target DNA sequences

  • Authors:
    • R Gambari
    • N Bianchi
    • C Rutigliano
    • E Borsetti
    • M Tomassetti
    • G Feriotto
    • F Zorzato
  • View Affiliations

  • Published online on: July 1, 1997     https://doi.org/10.3892/ijo.11.1.145
  • Pages: 145-149
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Abstract

DNA-binding drugs interfere with the activity of a large variety of transcription factors, leading to an alteration of transcription. This and similar effects could have important practical applications in the experimental therapy of many human pathologies, including neoplastic diseases. The analysis of sequence selectivity of DNA-binding drugs by footprinting, gel retardation studies, polymerase chain reaction and in vitro transcription does not allow an easy study of kinetics of binding and dissociation. The recent development of biosensor technologies for biospecific interaction analysis (BIA) enables the monitoring of a variety of molecular reactions in real-time by surface plasmon resonance (SPR). In this report we demonstrate that molecular interactions between the DNA-binding drug chromomycin and a biotinylated GC-rich Ha-ras oligonucleotide probe immobilized on a sensor chip is detectable by SPR technology using the BIAcore(TM) biosensor. This approach appears of interest in the development of drugs exhibiting differential affinity for target DNA sequences for the following reasons: a) results are obtained within one hour; b) unlike footprinting and gel retardation studies, this technology does not require P-32-labelled probes; c) BIA allows kinetic studies of both association and dissociation.

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July 1997
Volume 11 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Gambari R, Bianchi N, Rutigliano C, Borsetti E, Tomassetti M, Feriotto G and Zorzato F: Surface plasmon resonance for real-time detection of molecular interactions between chromomycin and target DNA sequences. Int J Oncol 11: 145-149, 1997
APA
Gambari, R., Bianchi, N., Rutigliano, C., Borsetti, E., Tomassetti, M., Feriotto, G., & Zorzato, F. (1997). Surface plasmon resonance for real-time detection of molecular interactions between chromomycin and target DNA sequences. International Journal of Oncology, 11, 145-149. https://doi.org/10.3892/ijo.11.1.145
MLA
Gambari, R., Bianchi, N., Rutigliano, C., Borsetti, E., Tomassetti, M., Feriotto, G., Zorzato, F."Surface plasmon resonance for real-time detection of molecular interactions between chromomycin and target DNA sequences". International Journal of Oncology 11.1 (1997): 145-149.
Chicago
Gambari, R., Bianchi, N., Rutigliano, C., Borsetti, E., Tomassetti, M., Feriotto, G., Zorzato, F."Surface plasmon resonance for real-time detection of molecular interactions between chromomycin and target DNA sequences". International Journal of Oncology 11, no. 1 (1997): 145-149. https://doi.org/10.3892/ijo.11.1.145