Mutation and microsatellite instability analysis of the androgen receptor gene in human prostate cancer

  • Authors:
    • T Uchida
    • C Wang
    • T Sato
    • M Muramoto
    • M Iwamura
    • S Egawa
    • K Koshiba
  • View Affiliations

  • Published online on: September 1, 1997     https://doi.org/10.3892/ijo.11.3.551
  • Pages: 551-556
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

To investigate the mechanism for the development of human prostate cancer, examination was made of structural abnormality of the androgen receptor (AR) gene in 29 human prostate cancer. AR gene mutations from exons A to H were examined by PCR-SSCP and microsatellite instability analysis using (CAG)n and (GGN)n polymorphic markers in AR gene exon A. A point mutation was found in the exon D hormone-binding domain of AR leading to substitution of glutamine (GAG) for wild-type arginine (CGG) at codon 629 in 1 (3.4%) hormone-independent stage D2 patient. Microsatellite instability was detected in 5 of the 27 (18.5%) patients, 1 of 6 (16.7%) hormone-independent stage D2 and 4 of 21 (19.0%) hormone-dependent and non-treated prostate cancer patients. AR mutations may possibly be involved in the transition from androgen-dependent to independent stages during androgen ablation therapy.

Related Articles

Journal Cover

September 1997
Volume 11 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Uchida T, Wang C, Sato T, Muramoto M, Iwamura M, Egawa S and Koshiba K: Mutation and microsatellite instability analysis of the androgen receptor gene in human prostate cancer. Int J Oncol 11: 551-556, 1997
APA
Uchida, T., Wang, C., Sato, T., Muramoto, M., Iwamura, M., Egawa, S., & Koshiba, K. (1997). Mutation and microsatellite instability analysis of the androgen receptor gene in human prostate cancer. International Journal of Oncology, 11, 551-556. https://doi.org/10.3892/ijo.11.3.551
MLA
Uchida, T., Wang, C., Sato, T., Muramoto, M., Iwamura, M., Egawa, S., Koshiba, K."Mutation and microsatellite instability analysis of the androgen receptor gene in human prostate cancer". International Journal of Oncology 11.3 (1997): 551-556.
Chicago
Uchida, T., Wang, C., Sato, T., Muramoto, M., Iwamura, M., Egawa, S., Koshiba, K."Mutation and microsatellite instability analysis of the androgen receptor gene in human prostate cancer". International Journal of Oncology 11, no. 3 (1997): 551-556. https://doi.org/10.3892/ijo.11.3.551