Characterization and purification of angiogenic factor derived from highly liver metastatic colon cancer cells

  • Authors:
    • S Takatsuka
    • Y Chung
    • N Yamada
    • T Sawada
    • S Seki
    • Y Funae
    • M Sowa
  • View Affiliations

  • Published online on: November 1, 1997     https://doi.org/10.3892/ijo.11.5.1035
  • Pages: 1035-1040
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Abstract

We investigated the effects of serum-free conditioned medium (SFCM) of colon cancer cells on human umbilical vein endothelial cells (HUVECs). SFCM of highly liver metastatic cell line (LM-HS) strongly enhanced proliferation, migration and tube formation of HUVECs. Moreover, it was suggested that a main angiogenic factor derived from LM-H5 may be VEGF based on HPLC, SDS-PAGE analysis and neutralizing experiment using anti-growth factor antibodies. These findings suggest that angiogenesis is related to the processes of metastasis of colon cancer, and that VEGF synthesized by colon cancer cells acts as a key factor inducing angiogenesis.

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November 1997
Volume 11 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Takatsuka S, Chung Y, Yamada N, Sawada T, Seki S, Funae Y and Sowa M: Characterization and purification of angiogenic factor derived from highly liver metastatic colon cancer cells. Int J Oncol 11: 1035-1040, 1997
APA
Takatsuka, S., Chung, Y., Yamada, N., Sawada, T., Seki, S., Funae, Y., & Sowa, M. (1997). Characterization and purification of angiogenic factor derived from highly liver metastatic colon cancer cells. International Journal of Oncology, 11, 1035-1040. https://doi.org/10.3892/ijo.11.5.1035
MLA
Takatsuka, S., Chung, Y., Yamada, N., Sawada, T., Seki, S., Funae, Y., Sowa, M."Characterization and purification of angiogenic factor derived from highly liver metastatic colon cancer cells". International Journal of Oncology 11.5 (1997): 1035-1040.
Chicago
Takatsuka, S., Chung, Y., Yamada, N., Sawada, T., Seki, S., Funae, Y., Sowa, M."Characterization and purification of angiogenic factor derived from highly liver metastatic colon cancer cells". International Journal of Oncology 11, no. 5 (1997): 1035-1040. https://doi.org/10.3892/ijo.11.5.1035