Rapamycin-induced apoptosis is p53-independent in human prostate carcinoma PC-3 cells

  • Authors:
    • C Ahn
    • M Hwang
    • P Ramsamooj
    • S Lee
    • M Jung
  • View Affiliations

  • Published online on: November 1, 1997     https://doi.org/10.3892/ijo.11.5.1115
  • Pages: 1115-1118
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Abstract

We have investigated the mechanisms of rapamycin-induced growth inhibition and apoptosis in the PC-3 prostate carcinoma cell line. Rapamycin induced apoptosis as well as the expression of p21(waf1) mRNA and protein, independent of p53. Rapamycin treatment also resulted in: a decrease in cdk2 kinase activity; an increase in hypophosphorylated retinoblastoma protein (pRb); a dephosphorylation of p70 S6 kinase; and, growth-arrest in G(1)-phase of cell cycle. These data suggest that rapamycin-induced growth arrest and apoptosis occur through the p53-independent induction of p21(waf1). Since this induction occurred soon after rapamycin treatment, possibly, the early induction of p21(waf1) and G(1)-arrest are important components of the mechanism by which rapamycin induces apoptosis in PC-3 cells.

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November 1997
Volume 11 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ahn C, Hwang M, Ramsamooj P, Lee S and Jung M: Rapamycin-induced apoptosis is p53-independent in human prostate carcinoma PC-3 cells. Int J Oncol 11: 1115-1118, 1997.
APA
Ahn, C., Hwang, M., Ramsamooj, P., Lee, S., & Jung, M. (1997). Rapamycin-induced apoptosis is p53-independent in human prostate carcinoma PC-3 cells. International Journal of Oncology, 11, 1115-1118. https://doi.org/10.3892/ijo.11.5.1115
MLA
Ahn, C., Hwang, M., Ramsamooj, P., Lee, S., Jung, M."Rapamycin-induced apoptosis is p53-independent in human prostate carcinoma PC-3 cells". International Journal of Oncology 11.5 (1997): 1115-1118.
Chicago
Ahn, C., Hwang, M., Ramsamooj, P., Lee, S., Jung, M."Rapamycin-induced apoptosis is p53-independent in human prostate carcinoma PC-3 cells". International Journal of Oncology 11, no. 5 (1997): 1115-1118. https://doi.org/10.3892/ijo.11.5.1115