Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Oncology
Join Editorial Board Propose a Special Issue
Print ISSN: 1019-6439 Online ISSN: 1791-2423
Journal Cover
December 1997 Volume 11 Issue 6

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December 1997 Volume 11 Issue 6

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Purchase PDF
Article

Analysis of K-ras, p53, bcl-2 and Rb expression in non-small cell lung cancer cell lines

  • Authors:
    • M Loprevite
    • L Varesco
    • R Favoni
    • G Ferrara
    • F Moro
    • L Ottaggio
    • G Fronza
    • P Campomenosi
    • A Abbondandolo
    • G Cutrona
    • S Roncella
    • A Albini
    • M Aluigi
    • S Pozzi
    • C Pera
    • R Biticchi
    • V Gismondi
    • F Grossi
    • M Pennucci
    • A Ardizzoni
  • View Affiliations / Copyright

    Affiliations: IST NAZL RIC CANC,DEPT MED ONCOL 1,CTR BIOTECNOL AVANZANTE,I-16132 GENOA,ITALY. IST NAZL RIC CANC,DEPT IMMUNOGENET,CTR BIOTECNOL AVANZANTE,I-16132 GENOA,ITALY. IST NAZL RIC CANC,DEPT EXPT PHARMACOL,CTR BIOTECNOL AVANZANTE,I-16132 GENOA,ITALY. IST NAZL RIC CANC,DEPT MUTAGENESIS,CTR BIOTECNOL AVANZANTE,I-16132 GENOA,ITALY. IST NAZL RIC CANC,DEPT CLIN IMMUNOL,CTR BIOTECNOL AVANZANTE,I-16132 GENOA,ITALY. IST NAZL RIC CANC,DEPT EXPT ONCOL,CTR BIOTECNOL AVANZANTE,I-16132 GENOA,ITALY.
  • Pages: 1203-1208
    |
    Published online on: December 1, 1997
       https://doi.org/10.3892/ijo.11.6.1203
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Six non-small cell lung cancer (NSCLC) cell lines (A-549, Ca-Lu-6, SK-Lu-1, Ca-Lu-1, SK-Mes-1 and LX-1) were studied to assess the presence of multiple concomitant alterations of different oncogenes (K-ras, bcl-2) and tumor suppressor genes (p53, Rb) in NSCLC. K-ras (exon 1) and p53 (exons 5-8) gene mutations were determined via a PCR-based-DGGE (Denaturing Gradient Gel Electro-phoresis) and by sequencing approach. Different mutations were found in the Ist exon of K-ras gene in 5 of 6 cell lines examined. Five of six cell lines contained K-ras mutations at codon 12 (A-549, SK-Lu-1, LX-1) or codon 13 (SK-Mes-1, Ca-Lu-1). In addition, 5 of 6 cell lines showed p53 mutations of exon 8 (SK-Mes-1, Ca-Lu-1 cod. 280; LX-1 cod. 273) or exon 6 (Ca-Lu-6 cod. 196; SK-Lu-1 cod. 193). In 4 of these cell lines, p53 protein nuclear expression was also confirmed with DO-7 mAb immunocytochemistry. Expression of cytoplasmic bcl-2 protein, by anti-bcl-2 mAb flow cytometric analysis, was found in A-549, Ca-Lu-1, SK-Lu-1, SK-Mes-1 cell lines. In contrast, RT-PCR analysis of Rb gene could not identify any change in the cell lines examined. In conclusion, most NSCLC cell lines tested displayed concomitant multiple oncogene/tumor suppressor gene alterations.

Related Articles

  • Abstract
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Loprevite M, Varesco L, Favoni R, Ferrara G, Moro F, Ottaggio L, Fronza G, Campomenosi P, Abbondandolo A, Cutrona G, Cutrona G, et al: Analysis of K-ras, p53, bcl-2 and Rb expression in non-small cell lung cancer cell lines. Int J Oncol 11: 1203-1208, 1997.
APA
Loprevite, M., Varesco, L., Favoni, R., Ferrara, G., Moro, F., Ottaggio, L. ... Ardizzoni, A. (1997). Analysis of K-ras, p53, bcl-2 and Rb expression in non-small cell lung cancer cell lines. International Journal of Oncology, 11, 1203-1208. https://doi.org/10.3892/ijo.11.6.1203
MLA
Loprevite, M., Varesco, L., Favoni, R., Ferrara, G., Moro, F., Ottaggio, L., Fronza, G., Campomenosi, P., Abbondandolo, A., Cutrona, G., Roncella, S., Albini, A., Aluigi, M., Pozzi, S., Pera, C., Biticchi, R., Gismondi, V., Grossi, F., Pennucci, M., Ardizzoni, A."Analysis of K-ras, p53, bcl-2 and Rb expression in non-small cell lung cancer cell lines". International Journal of Oncology 11.6 (1997): 1203-1208.
Chicago
Loprevite, M., Varesco, L., Favoni, R., Ferrara, G., Moro, F., Ottaggio, L., Fronza, G., Campomenosi, P., Abbondandolo, A., Cutrona, G., Roncella, S., Albini, A., Aluigi, M., Pozzi, S., Pera, C., Biticchi, R., Gismondi, V., Grossi, F., Pennucci, M., Ardizzoni, A."Analysis of K-ras, p53, bcl-2 and Rb expression in non-small cell lung cancer cell lines". International Journal of Oncology 11, no. 6 (1997): 1203-1208. https://doi.org/10.3892/ijo.11.6.1203
Copy and paste a formatted citation
x
Spandidos Publications style
Loprevite M, Varesco L, Favoni R, Ferrara G, Moro F, Ottaggio L, Fronza G, Campomenosi P, Abbondandolo A, Cutrona G, Cutrona G, et al: Analysis of K-ras, p53, bcl-2 and Rb expression in non-small cell lung cancer cell lines. Int J Oncol 11: 1203-1208, 1997.
APA
Loprevite, M., Varesco, L., Favoni, R., Ferrara, G., Moro, F., Ottaggio, L. ... Ardizzoni, A. (1997). Analysis of K-ras, p53, bcl-2 and Rb expression in non-small cell lung cancer cell lines. International Journal of Oncology, 11, 1203-1208. https://doi.org/10.3892/ijo.11.6.1203
MLA
Loprevite, M., Varesco, L., Favoni, R., Ferrara, G., Moro, F., Ottaggio, L., Fronza, G., Campomenosi, P., Abbondandolo, A., Cutrona, G., Roncella, S., Albini, A., Aluigi, M., Pozzi, S., Pera, C., Biticchi, R., Gismondi, V., Grossi, F., Pennucci, M., Ardizzoni, A."Analysis of K-ras, p53, bcl-2 and Rb expression in non-small cell lung cancer cell lines". International Journal of Oncology 11.6 (1997): 1203-1208.
Chicago
Loprevite, M., Varesco, L., Favoni, R., Ferrara, G., Moro, F., Ottaggio, L., Fronza, G., Campomenosi, P., Abbondandolo, A., Cutrona, G., Roncella, S., Albini, A., Aluigi, M., Pozzi, S., Pera, C., Biticchi, R., Gismondi, V., Grossi, F., Pennucci, M., Ardizzoni, A."Analysis of K-ras, p53, bcl-2 and Rb expression in non-small cell lung cancer cell lines". International Journal of Oncology 11, no. 6 (1997): 1203-1208. https://doi.org/10.3892/ijo.11.6.1203
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team