Polymerase chain reaction amplification of long DNA targets: application to analysis of chromosomal translocations in human B-cell tumors (review).

  • Authors:
    • T Akasaka
    • H Akasaka
    • H Ohno
  • View Affiliations

  • Published online on: January 1, 1998     https://doi.org/10.3892/ijo.12.1.113
  • Pages: 113-134
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Abstract

Chromosomal translocations in human leukemias generate fusion transcripts containing messages from two genes involved in the translocation, and these have been the targets for reverse transcriptase-mediated polymerase chain reaction (PCR). In contrast, many of the translocations in B-cell tumors involve immunoglobulin gene (IG) loci, and coding regions of the oncogenes on partner chromosomes are not interrupted by the translocation. Therefore, targets for PCR amplification are single-copy oncogene/IG fusion sequences within the complex genomic DNA. We present here a novel strategy for detection of translocations in B-cell tumors on the basis of long-distance (LD-) PCR that is capable of amplifying up to 30 kb of DNA. LD-PCR is a general method using primer pairs designed for distinctive regions of IG and oncogenes involved in translocations, and amplifying long DNA fragments encompassing the oncogene/IG junction. LD-PCR is capable of detecting virtually all the important translocations in B-cell tumors, including t(8;14)(q24;q32), t(14;18)(q32;q21), t(3;14)(q27;q32) and its variants. We show here that LD-PCR can substitute for time-consuming Southern blot hybridization in the rapid detection of these translocations. Furthermore, as amplified fragments obtained by LD-PCR contained exons and flanking sequences of the oncogenes and IGs, restriction analysis and nucleotide sequencing of the products refined the characteristics of translocations.

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Jan 1998
Volume 12 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Akasaka T, Akasaka H and Ohno H: Polymerase chain reaction amplification of long DNA targets: application to analysis of chromosomal translocations in human B-cell tumors (review).. Int J Oncol 12: 113-134, 1998
APA
Akasaka, T., Akasaka, H., & Ohno, H. (1998). Polymerase chain reaction amplification of long DNA targets: application to analysis of chromosomal translocations in human B-cell tumors (review).. International Journal of Oncology, 12, 113-134. https://doi.org/10.3892/ijo.12.1.113
MLA
Akasaka, T., Akasaka, H., Ohno, H."Polymerase chain reaction amplification of long DNA targets: application to analysis of chromosomal translocations in human B-cell tumors (review).". International Journal of Oncology 12.1 (1998): 113-134.
Chicago
Akasaka, T., Akasaka, H., Ohno, H."Polymerase chain reaction amplification of long DNA targets: application to analysis of chromosomal translocations in human B-cell tumors (review).". International Journal of Oncology 12, no. 1 (1998): 113-134. https://doi.org/10.3892/ijo.12.1.113