CDKN2/p16 inactivation and p16 immunohistochemistry in astrocytic gliomas.

  • Authors:
    • R Piva
    • P Cavalla
    • S Bortolotto
    • S Cordera
    • R Grosso
    • P Richiardi
    • A Dutto
    • D Schiffer
  • View Affiliations

  • Published online on: January 1, 1998     https://doi.org/10.3892/ijo.12.1.55
  • Pages: 55-63
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Abstract

CDKN2/p16 inactivation is the most frequent alteration in the molecular regulation of G1-S transition. CDKN2/p16 homozygous deletions was studied in paraffin-embedded sections of 45 astrocytic tumours by multiplex PCR. Immunohistochemistry for p16 and proliferation marker Ki-67 MIB-1 was performed in adjacent sections; their labelling index (LI) have been calculated. CDKN2/p16 gene was not deleted in astrocytomas, while homozygous deletion was found in 26.7% anaplastic astrocytomas, and in 55.0% of glioblastomas. Analysis of CDKN2/p16 homozygous deletion in discrete areas of the same tumour, showed that the deletion occurred independently of the phenotypic aspect of the areas. Nevertheless a genotypic and phenotypic heterogeneity is present in few cases. p16 immunohistochemistry mostly corresponds to the genotypic pattern. No correlation was found between CDKN2/p16 homozygous deletion and MIB-1 LI.

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Jan 1998
Volume 12 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Piva R, Cavalla P, Bortolotto S, Cordera S, Grosso R, Richiardi P, Dutto A and Schiffer D: CDKN2/p16 inactivation and p16 immunohistochemistry in astrocytic gliomas.. Int J Oncol 12: 55-63, 1998
APA
Piva, R., Cavalla, P., Bortolotto, S., Cordera, S., Grosso, R., Richiardi, P. ... Schiffer, D. (1998). CDKN2/p16 inactivation and p16 immunohistochemistry in astrocytic gliomas.. International Journal of Oncology, 12, 55-63. https://doi.org/10.3892/ijo.12.1.55
MLA
Piva, R., Cavalla, P., Bortolotto, S., Cordera, S., Grosso, R., Richiardi, P., Dutto, A., Schiffer, D."CDKN2/p16 inactivation and p16 immunohistochemistry in astrocytic gliomas.". International Journal of Oncology 12.1 (1998): 55-63.
Chicago
Piva, R., Cavalla, P., Bortolotto, S., Cordera, S., Grosso, R., Richiardi, P., Dutto, A., Schiffer, D."CDKN2/p16 inactivation and p16 immunohistochemistry in astrocytic gliomas.". International Journal of Oncology 12, no. 1 (1998): 55-63. https://doi.org/10.3892/ijo.12.1.55