Multiple cell populations in colorectal carcinomas: analysis by 3-colour fluorescence in situ hybridization.

Greenman,J; Ashman,J N; Brankin,V; McDonald,A W; Duthie,G S; Lee,P W; Kerin,M J; Monson,J R

doi:10.3892/ijo.12.1.75

Jan 1998 Volume 12 Issue 1

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Jan 1998 Volume 12 Issue 1

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Article

Multiple cell populations in colorectal carcinomas: analysis by 3-colour fluorescence in situ hybridization.

Authors:
- J Greenman
- J N Ashman
- V Brankin
- A W McDonald
- G S Duthie
- P W Lee
- M J Kerin
- J R Monson
View Affiliations / Copyright

Affiliations: Academic Surgical Unit, University of Hull, Medical Research Laboratory, Wolfson Building, Hull, HU6 7RX, UK.
Pages: 75-155
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Published online on: January 1, 1998

https://doi.org/10.3892/ijo.12.1.75
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Abstract

A three-colour FISH approach using centromere-specific DNA probes was used to analyse the number of chromosomes 7, 17 and 18 found within individual tumour cells and the results were correlated with total DNA ploidy determined by image analysis. FISH analysis showed a high level of heterogeneity in the majority of tumour samples with only 7 out of 44 samples having a single chromosome profile occurring in greater than 40% of the cells. Analysis of the modal chromosome number showed that a diploid 2/2/2 profile for chromosome 7, 17 and 18 respectively occurred most commonly. The DNA ploidy index for biopsies with a 2/2/2 profile varied between 0.93-2.06. No gain of chromosome was observed in the adenoma samples or Dukes A tumours but a loss of chromosome 18 was seen in 50% of these early carcinomas. A modal chromosome profile of 4/2/2 was commonly found in Dukes B and C tumours suggesting that endoreduplication with the relative loss of chromosome 17 and 18 is common in advanced cancers. The DNA ploidy index for the more advanced tumours was also variable but significantly higher than that found in the early tumours and non-tumour controls. In conclusion, this work shows that tumours are highly heterogeneous and that the majority of tumours consist of a large number of cell sub-populations with respect to the expression of chromosomes 7, 17 and 18.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Multiple cell populations in colorectal carcinomas: analysis by 3-colour fluorescence in situ hybridization.

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Abstract

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