Effects of short-term pre-operative tamoxifen on steroid receptor and Ki-67 expression in primary breast cancer: an immunocytochemical study.

  • Authors:
    • E Bajetta
    • L Celio
    • A Di Leo
    • C Bartoli
    • S Pilotti
    • M Leutner
    • A Bono
    • L Ferrari
    • R Buzzoni
    • N Zilembo
    • D De Candis
    • D Moglia
  • View Affiliations

  • Published online on: April 1, 1998     https://doi.org/10.3892/ijo.12.4.853
  • Pages: 853-861
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Abstract

The effects of the short-term pre-operative administration of tamoxifen (TAM, 20 mg once daily) on the tumor levels of steroid receptors and the nuclear proliferation Ki-67 antigen, were investigated in 32 elderly patients with hormone-sensitive, operable primary breast cancer by means of fine-needle aspiration biopsy (FNAB). The FNAB smears before (pre-TAM) and after six weeks of treatment (post-TAM) were stained immunocytochemically in order to obtain an H-score for steroid receptors, and the percentage of cellular nuclei containing Ki-67. The mean oestrogen receptor (ER) score between the pre- and post-TAM specimens fell from 181.2 9.7 ( SEM) to 148.1 7.9 (Wilcoxon's matched-pairs signed-rank test, p=0. 01) and there was also a significant decrease in both the mean progesterone receptor (PgR) score (178.4 10.6 vs 148.5 10.6; p=0.01) and mean Ki-67 index (8.2% 1.2 vs 4.9% 0.9; p=0.0002). The reliability of FNAB as a sampling method was checked by comparing the results of the immunocytochemical assay (ICA) of the post-TAM biopsies with those of the immunohistochemical assay (IHA) of the corresponding excised tumors. There was a positive correlation between the ICA and IHA scores: ER (Spearman's correlation coefficient, rho=0.66, p<0.001), PgR (rho=0.84, p<0.001) and Ki-67 (rho=0.96, p<0.001). We conclude that the sequential use of FNAB is a reliable means of assessing the behaviour of within-tumor biomarkers during endocrine therapy.

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Apr 1998
Volume 12 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Bajetta E, Celio L, Di Leo A, Bartoli C, Pilotti S, Leutner M, Bono A, Ferrari L, Buzzoni R, Zilembo N, Zilembo N, et al: Effects of short-term pre-operative tamoxifen on steroid receptor and Ki-67 expression in primary breast cancer: an immunocytochemical study.. Int J Oncol 12: 853-861, 1998.
APA
Bajetta, E., Celio, L., Di Leo, A., Bartoli, C., Pilotti, S., Leutner, M. ... Moglia, D. (1998). Effects of short-term pre-operative tamoxifen on steroid receptor and Ki-67 expression in primary breast cancer: an immunocytochemical study.. International Journal of Oncology, 12, 853-861. https://doi.org/10.3892/ijo.12.4.853
MLA
Bajetta, E., Celio, L., Di Leo, A., Bartoli, C., Pilotti, S., Leutner, M., Bono, A., Ferrari, L., Buzzoni, R., Zilembo, N., De Candis, D., Moglia, D."Effects of short-term pre-operative tamoxifen on steroid receptor and Ki-67 expression in primary breast cancer: an immunocytochemical study.". International Journal of Oncology 12.4 (1998): 853-861.
Chicago
Bajetta, E., Celio, L., Di Leo, A., Bartoli, C., Pilotti, S., Leutner, M., Bono, A., Ferrari, L., Buzzoni, R., Zilembo, N., De Candis, D., Moglia, D."Effects of short-term pre-operative tamoxifen on steroid receptor and Ki-67 expression in primary breast cancer: an immunocytochemical study.". International Journal of Oncology 12, no. 4 (1998): 853-861. https://doi.org/10.3892/ijo.12.4.853