Microsatellite instability, chemosensitivity and mutant frequency in a series of 1,2-dimethylhydrazine induced murine colon adenocarcinoma models.

  • Authors:
    • M J Thompson
    • R Slack
    • J A Double
    • M C Bibby
  • View Affiliations

  • Published online on: September 1, 1998     https://doi.org/10.3892/ijo.13.3.531
  • Pages: 531-537
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Abstract

Loss of DNA mismatch repair has been described in a number of tumour types such as colorectal adenocarcinoma and leads to microsatellite instability. This may have clinical relevance due to mismatch repair defects altering chemosensitivity towards certain classes of anti-tumour agent. This study has examined microsatellite instability of eight murine colon adenocarcinoma tumour models induced by 1,2-dimethylhydrazine. Four microsatellite regions were examined suggesting that four of the tumour models exhibit a low level of microsatellite instability. Loss of heterozygosity was found in 5/8 tumours, suggesting that allelic loss may be a relatively common step in the carcinogenesis of these tumour models. Three of the allelic losses involved the D11MIT4 locus which is situated very close to the p53 tumour suppressor locus. Four tumour models are routinely cultured in vitro and these were used to examine whether there was any association between microsatellite instability, mutant frequency and chemo-sensitivity of these tumour models, comparing them with four human adenocarcinoma cell lines of known mismatch repair status. Two cell lines (MAC26 and MAC16) were found to be more chemoresistant towards cisplatin but not 6-thioguanine. No association was found between microsatellite instability and chemosensitivity for either the human or mouse cell lines.

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Sep 1998
Volume 13 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Thompson M, Slack R, Double J and Bibby M: Microsatellite instability, chemosensitivity and mutant frequency in a series of 1,2-dimethylhydrazine induced murine colon adenocarcinoma models.. Int J Oncol 13: 531-537, 1998
APA
Thompson, M., Slack, R., Double, J., & Bibby, M. (1998). Microsatellite instability, chemosensitivity and mutant frequency in a series of 1,2-dimethylhydrazine induced murine colon adenocarcinoma models.. International Journal of Oncology, 13, 531-537. https://doi.org/10.3892/ijo.13.3.531
MLA
Thompson, M., Slack, R., Double, J., Bibby, M."Microsatellite instability, chemosensitivity and mutant frequency in a series of 1,2-dimethylhydrazine induced murine colon adenocarcinoma models.". International Journal of Oncology 13.3 (1998): 531-537.
Chicago
Thompson, M., Slack, R., Double, J., Bibby, M."Microsatellite instability, chemosensitivity and mutant frequency in a series of 1,2-dimethylhydrazine induced murine colon adenocarcinoma models.". International Journal of Oncology 13, no. 3 (1998): 531-537. https://doi.org/10.3892/ijo.13.3.531