Expression of c-MET/HGF receptor mRNA and protein in human non-malignant and malignant prostate tissues.

  • Authors:
    • H Tsuka
    • H Mori
    • B Li
    • H Kanamaru
    • S Matsukawa
    • K Okada
  • View Affiliations

  • Published online on: November 1, 1998     https://doi.org/10.3892/ijo.13.5.927
  • Pages: 927-961
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Abstract

We previously reported the expression of hepatocyte growth factor (HGF), a ligand for c-MET, at the level of mRNA and protein in human prostate tissues. The present study was carried out to evaluate the relationship between c-MET expression and cancer cell proliferation or effect of cancer therapy. The expression both in mRNA and protein levels of c-MET proto-oncogene was determined semi-quantitatively by reverse transcription-competitive polymerase chain reaction (RT-competitive PCR) and Western blot analysis for prostate tissues from 32 Japanese male subjects. In addition, tissue localization of c-MET translation product was examined by immunohistochemistry for corresponding specimens. Although there was significantly higher c-MET protein expression in malignant (prostate cancer treated with/without neoadjuvant endocrine therapy) than in non-malignant prostate tissues (normal prostate and benign prostate hyperplasia; BPH), unexpectedly, c-MET mRNA showed high expression in the non-malignant group. Thus, there was no parallelism between mRNA and protein expressions of c-MET. Endocrine therapy did not alter c-MET mRNA and protein expressions in human prostate cancer. Immunohistochemical localization and expression of c-MET protein was found to be intense in cancer cells and weak in epithelia of normal and hyperplastic prostates. Unconcerted expression of mRNA and protein of c-MET, the reason of which is uninterpretable, is supposed to be one of characteristics of human prostate cancer.

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November 1998
Volume 13 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Tsuka, H., Mori, H., Li, B., Kanamaru, H., Matsukawa, S., & Okada, K. (1998). Expression of c-MET/HGF receptor mRNA and protein in human non-malignant and malignant prostate tissues.. International Journal of Oncology, 13, 927-961. https://doi.org/10.3892/ijo.13.5.927
MLA
Tsuka, H., Mori, H., Li, B., Kanamaru, H., Matsukawa, S., Okada, K."Expression of c-MET/HGF receptor mRNA and protein in human non-malignant and malignant prostate tissues.". International Journal of Oncology 13.5 (1998): 927-961.
Chicago
Tsuka, H., Mori, H., Li, B., Kanamaru, H., Matsukawa, S., Okada, K."Expression of c-MET/HGF receptor mRNA and protein in human non-malignant and malignant prostate tissues.". International Journal of Oncology 13, no. 5 (1998): 927-961. https://doi.org/10.3892/ijo.13.5.927