Improvement in detecting telomerase activity using silica-based resin treatment: An experience of urine in bladder carcinoma.

  • Authors:
    • N Yahata
    • K Nagao
    • J H Ohyashiki
    • H Hisatomi
    • K Ohyashiki
  • View Affiliations

  • Published online on: April 1, 1999     https://doi.org/10.3892/ijo.14.4.709
  • Pages: 709-721
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Abstract

To improve the telomeric repeat amplification protocol (TRAP) assay to detect telomerase activity using a small amount of sample, we used a resin-column to purify and to concentrate the TS extension DNA sequence. We used 14 samples of naturally voided urine (10 ml) from patients with bladder carcinoma and 9 urine samples from patients with non-malignant urological neoplasias. We used ethylenediamine tetraacetic acid (EDTA) to stabilize telomerase activity and resin treatment to concentrate TS-extended DNA and to exclude PCR inhibitor(s), and then performed extract-based fluorescence TRAP to detect telomerase activity. None of the urinary samples without resin-column treatment had detectable telomerase activity, whereas, in resin-column treated samples, 4/9 (44%) urine samples without EDTA and 9/14 (64%) with EDTA treatment had detectable telomerase activity. A combination of EDTA treatment and resin-column thus may be available to detect telomerase activity using a relatively small amount of secretion fluids, including exfoliated urinary cells.

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Apr 1999
Volume 14 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Yahata N, Nagao K, Ohyashiki J, Hisatomi H and Ohyashiki K: Improvement in detecting telomerase activity using silica-based resin treatment: An experience of urine in bladder carcinoma.. Int J Oncol 14: 709-721, 1999
APA
Yahata, N., Nagao, K., Ohyashiki, J., Hisatomi, H., & Ohyashiki, K. (1999). Improvement in detecting telomerase activity using silica-based resin treatment: An experience of urine in bladder carcinoma.. International Journal of Oncology, 14, 709-721. https://doi.org/10.3892/ijo.14.4.709
MLA
Yahata, N., Nagao, K., Ohyashiki, J., Hisatomi, H., Ohyashiki, K."Improvement in detecting telomerase activity using silica-based resin treatment: An experience of urine in bladder carcinoma.". International Journal of Oncology 14.4 (1999): 709-721.
Chicago
Yahata, N., Nagao, K., Ohyashiki, J., Hisatomi, H., Ohyashiki, K."Improvement in detecting telomerase activity using silica-based resin treatment: An experience of urine in bladder carcinoma.". International Journal of Oncology 14, no. 4 (1999): 709-721. https://doi.org/10.3892/ijo.14.4.709