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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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Aug 1999 Volume 15 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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Aug 1999 Volume 15 Issue 2

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Article

Comparative analysis of HPLC profile of cytosolic thymidine kinase activity between hepatoma and regenerating liver in rat with reference to phosphorylation.

  • Authors:
    • L Lin
    • M M Islam
    • M Du
    • Y Ohmura
    • T Hiwasa
    • S Fujimura

  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, Chiba University School of Medicine, Chuo-ku, Chiba 260-8670, Japan.
  • Pages: 293-302
    |
    Published online on: August 1, 1999
       https://doi.org/10.3892/ijo.15.2.293
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Abstract

Thymidine kinase (TK) activity in rat hepatoma JB1 and AH136B was mainly due to cytosolic TK and was much higher than that in rat regenerating liver. Two forms of cytosolic TK, designated as TK-I and TK-II, were revealed in addition to mitochondrial TK in anion-exchange high performance liquid chromatography (HPLC) of the enzyme extract from hepatoma JB1. During incubation of the enzyme extract at 20 degrees C in the presence of phosphatase inhibitor NaF, TK-II activity remained while TK-I activity almost disappeared. Thus, TK-II appeared to be more stable than TK-I. In Western blot analysis, TK-II was much more phosphorylated and exhibiting higher specific activity than TK-I. Meanwhile, regenerating liver derived from the rat 24 h after partial hepatectomy showed only TK-I activity, which completely disappeared after incubation at 20 degrees C even in the presence of NaF. Consequently, the presence of TK-II might account for the higher TK activity in hepatomas than in regenerating liver.

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Copy and paste a formatted citation
Spandidos Publications style
Lin L, Islam M, Du M, Ohmura Y, Hiwasa T and Fujimura S: Comparative analysis of HPLC profile of cytosolic thymidine kinase activity between hepatoma and regenerating liver in rat with reference to phosphorylation.. Int J Oncol 15: 293-302, 1999.
APA
Lin, L., Islam, M., Du, M., Ohmura, Y., Hiwasa, T., & Fujimura, S. (1999). Comparative analysis of HPLC profile of cytosolic thymidine kinase activity between hepatoma and regenerating liver in rat with reference to phosphorylation.. International Journal of Oncology, 15, 293-302. https://doi.org/10.3892/ijo.15.2.293
MLA
Lin, L., Islam, M., Du, M., Ohmura, Y., Hiwasa, T., Fujimura, S."Comparative analysis of HPLC profile of cytosolic thymidine kinase activity between hepatoma and regenerating liver in rat with reference to phosphorylation.". International Journal of Oncology 15.2 (1999): 293-302.
Chicago
Lin, L., Islam, M., Du, M., Ohmura, Y., Hiwasa, T., Fujimura, S."Comparative analysis of HPLC profile of cytosolic thymidine kinase activity between hepatoma and regenerating liver in rat with reference to phosphorylation.". International Journal of Oncology 15, no. 2 (1999): 293-302. https://doi.org/10.3892/ijo.15.2.293
Copy and paste a formatted citation
x
Spandidos Publications style
Lin L, Islam M, Du M, Ohmura Y, Hiwasa T and Fujimura S: Comparative analysis of HPLC profile of cytosolic thymidine kinase activity between hepatoma and regenerating liver in rat with reference to phosphorylation.. Int J Oncol 15: 293-302, 1999.
APA
Lin, L., Islam, M., Du, M., Ohmura, Y., Hiwasa, T., & Fujimura, S. (1999). Comparative analysis of HPLC profile of cytosolic thymidine kinase activity between hepatoma and regenerating liver in rat with reference to phosphorylation.. International Journal of Oncology, 15, 293-302. https://doi.org/10.3892/ijo.15.2.293
MLA
Lin, L., Islam, M., Du, M., Ohmura, Y., Hiwasa, T., Fujimura, S."Comparative analysis of HPLC profile of cytosolic thymidine kinase activity between hepatoma and regenerating liver in rat with reference to phosphorylation.". International Journal of Oncology 15.2 (1999): 293-302.
Chicago
Lin, L., Islam, M., Du, M., Ohmura, Y., Hiwasa, T., Fujimura, S."Comparative analysis of HPLC profile of cytosolic thymidine kinase activity between hepatoma and regenerating liver in rat with reference to phosphorylation.". International Journal of Oncology 15, no. 2 (1999): 293-302. https://doi.org/10.3892/ijo.15.2.293
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