EXTL3/EXTR1 alterations in colorectal cancer cell lines.

  • Authors:
    • T Arai
    • Y Akiyama
    • H Nagasaki
    • N Murase
    • S Okabe
    • T Ikeuchi
    • K Saito
    • T Iwai
    • Y Yuasa
  • View Affiliations

  • Published online on: November 1, 1999     https://doi.org/10.3892/ijo.15.5.915
  • Pages: 915-924
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Abstract

We previously demonstrated that metastasis-related tumor suppressor gene(s) may exist on chromosome 8p21-22 on allelotype analysis of early colorectal carcinomas (CRC) with lymph node metastasis. Here, we searched for target gene(s) in this chromosomal region in the UniGene database. The EXTL3 (also called EXTR1) gene was selected as a candidate because of its homology to EXT1 and EXT2, putative tumor suppressor genes. We screened 12 CRC cell lines for mutations by means of polymerase chain reaction (PCR)-single strand conformation polymorphism. Three cell lines showed EXTL3 mutations, all of which were located within exon 3 and caused amino acid substitutions. Reverse transcription-PCR analysis showed that the EXTL3 expression was lacking in 1 of the 12 colorectal cancer cell lines. Although there is still no definitive evidence that EXTL3 is a tumor suppressor gene for CRC, these data suggest that inactivation of the EXTL3 gene may at least offer a selective growth advantage for some CRC cell lines.

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Nov 1999
Volume 15 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Arai T, Akiyama Y, Nagasaki H, Murase N, Okabe S, Ikeuchi T, Saito K, Iwai T and Yuasa Y: EXTL3/EXTR1 alterations in colorectal cancer cell lines.. Int J Oncol 15: 915-924, 1999
APA
Arai, T., Akiyama, Y., Nagasaki, H., Murase, N., Okabe, S., Ikeuchi, T. ... Yuasa, Y. (1999). EXTL3/EXTR1 alterations in colorectal cancer cell lines.. International Journal of Oncology, 15, 915-924. https://doi.org/10.3892/ijo.15.5.915
MLA
Arai, T., Akiyama, Y., Nagasaki, H., Murase, N., Okabe, S., Ikeuchi, T., Saito, K., Iwai, T., Yuasa, Y."EXTL3/EXTR1 alterations in colorectal cancer cell lines.". International Journal of Oncology 15.5 (1999): 915-924.
Chicago
Arai, T., Akiyama, Y., Nagasaki, H., Murase, N., Okabe, S., Ikeuchi, T., Saito, K., Iwai, T., Yuasa, Y."EXTL3/EXTR1 alterations in colorectal cancer cell lines.". International Journal of Oncology 15, no. 5 (1999): 915-924. https://doi.org/10.3892/ijo.15.5.915