Adriamycin activates E-cadherin-mediated cell-cell adhesion in human breast cancer cells.

  • Authors:
    • S Z Yang
    • N Kohno
    • K Kondo
    • A Yokoyama
    • H Hamada
    • K Hiwada
    • M Miyake
  • View Affiliations

  • Published online on: December 1, 1999     https://doi.org/10.3892/ijo.15.6.1109
  • Pages: 1109-1124
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Abstract

E-cadherin-mediated cell-cell adhesion plays a crucial role in intercellular communication, which is related to the regulation of cell proliferation, differentiation, and apoptosis. Our previous study showed that decreased expression of MUC1 can induce E-cadherin-mediated cell-cell adhesion in human breast cancer cell lines proliferating in suspension without aggregation. Using such a cell line (YMB-S), we observed the effects of an anticancer agent, adriamycin, on cell-cell adhesion and expression of E-cadherin-catenin complex and MUC1. The cells showed E-cadherin-mediated cell-cell adhesion after 48 h exposure to 0.4 micromol/l adriamycin. And in these cells, expression of E-cadherin and beta-catenin mRNA obviously began to increase, while expression of MUC1 mRNA decreased, as demonstrated by Northern blot analysis. Such change in mRNA levels were followed by increases in E-cadherin and beta-catenin protein levels and a decrease in MUC1 protein level. Though expression of alpha-catenin mRNA began to increase on day 2, its protein level did not change. In immunohistochemical analysis, beta-catenin protein in untreated cells showed diffuse cytoplasmic localization, whereas beta-catenin in treated cells was present in cytoplasm with a clear submembranous localization, indicating that increased beta-catenin mainly bound with E-cadherin, participating in cell-cell adhesion. These findings show for the first time that adriamycin can induce E-cadherin-mediated cell-cell adhesion by increasing expression of E-cadherin and beta-catenin and decreasing expression of MUC1 during breast cancer cell apoptosis induced by this drug.

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Dec 1999
Volume 15 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Yang S, Kohno N, Kondo K, Yokoyama A, Hamada H, Hiwada K and Miyake M: Adriamycin activates E-cadherin-mediated cell-cell adhesion in human breast cancer cells.. Int J Oncol 15: 1109-1124, 1999
APA
Yang, S., Kohno, N., Kondo, K., Yokoyama, A., Hamada, H., Hiwada, K., & Miyake, M. (1999). Adriamycin activates E-cadherin-mediated cell-cell adhesion in human breast cancer cells.. International Journal of Oncology, 15, 1109-1124. https://doi.org/10.3892/ijo.15.6.1109
MLA
Yang, S., Kohno, N., Kondo, K., Yokoyama, A., Hamada, H., Hiwada, K., Miyake, M."Adriamycin activates E-cadherin-mediated cell-cell adhesion in human breast cancer cells.". International Journal of Oncology 15.6 (1999): 1109-1124.
Chicago
Yang, S., Kohno, N., Kondo, K., Yokoyama, A., Hamada, H., Hiwada, K., Miyake, M."Adriamycin activates E-cadherin-mediated cell-cell adhesion in human breast cancer cells.". International Journal of Oncology 15, no. 6 (1999): 1109-1124. https://doi.org/10.3892/ijo.15.6.1109