Mutation and transcription analyses of the p63 gene in cervical carcinoma.

  • Authors:
    • H Nishi
    • K Isaka
    • Y Sagawa
    • S Usuda
    • A Fujito
    • H Ito
    • M Senoo
    • H Kato
    • M Takayama
  • View Affiliations

  • Published online on: December 1, 1999     https://doi.org/10.3892/ijo.15.6.1149
  • Pages: 1149-1202
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Abstract

Genetic mutation of p53, which monitors DNA damage and operates cellular checkpoints, is a major factor in the development of human malignancies. A novel gene p63/p73L/p51, encoding a protein with significant homology to p53 and p73, was recently identified at 3q27-9. To investigate the penetration of p63 in cervical carcinogenesis, mutation and transcription analyses of p63 were performed in cervical carcinoma. A certain isotype of p63 called TAp63gamma encodes the acidic N-terminus and possesses a short C-terminus. Using reverse transcriptase-polymerase chain reaction-single strand conformation polymorphism (RT-PCR-SSCP) analysis for TAp63gamma, one mutation was found in the cervical carcinoma cell line SKG-I. However, no mutations causing amino acid substitutions or frameshifts were found in 54 cases examined for TAp63gamma, which is thought to be a tumor suppressor gene. While cervical carcinomas tended to yield a positive signal in the RT-PCR reaction designed to amplify transcripts encoding the acidic N-terminus, normal cervix and cervical intraepithelial neoplasia (CIN) did not express this transcript. These data suggest that the p63 gene does not play an essential role as a tumor suppressor gene, but expression of TAp63gamma may be speculatively associated with tumor growth in cervical carcinogenesis.

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Dec 1999
Volume 15 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Nishi H, Isaka K, Sagawa Y, Usuda S, Fujito A, Ito H, Senoo M, Kato H and Takayama M: Mutation and transcription analyses of the p63 gene in cervical carcinoma.. Int J Oncol 15: 1149-1202, 1999
APA
Nishi, H., Isaka, K., Sagawa, Y., Usuda, S., Fujito, A., Ito, H. ... Takayama, M. (1999). Mutation and transcription analyses of the p63 gene in cervical carcinoma.. International Journal of Oncology, 15, 1149-1202. https://doi.org/10.3892/ijo.15.6.1149
MLA
Nishi, H., Isaka, K., Sagawa, Y., Usuda, S., Fujito, A., Ito, H., Senoo, M., Kato, H., Takayama, M."Mutation and transcription analyses of the p63 gene in cervical carcinoma.". International Journal of Oncology 15.6 (1999): 1149-1202.
Chicago
Nishi, H., Isaka, K., Sagawa, Y., Usuda, S., Fujito, A., Ito, H., Senoo, M., Kato, H., Takayama, M."Mutation and transcription analyses of the p63 gene in cervical carcinoma.". International Journal of Oncology 15, no. 6 (1999): 1149-1202. https://doi.org/10.3892/ijo.15.6.1149