Isolation of human colorectal tumour reactive antibodies using phage display technology.

  • Authors:
    • K P Topping
    • V C Hough
    • J R Monson
    • J Greenman
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  • Published online on: January 1, 2000     https://doi.org/10.3892/ijo.16.1.187
  • Pages: 187-282
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Abstract

Few highly specific anti-colorectal tumour antibodies exist. Here, we describe the isolation of a panel of colorectal tumour reactive antibodies, using bacteriophage-antibody display technology. A whole-cell based panning protocol has been used to isolate antibodies. panning against a cell type related to the colorectal tumour cells was used to remove antibodies reacting with common antigens. The 11 antibodies studied possess different levels of specificity, all bound to protein or glycoprotein molecules, and 2 of them recognised tunicamycin-sensitive epitopes. None of the antibodies recognise the commonly expressed colorectal-markers CEA or EP-CAM. Our work demonstrates the potential of this technology for the development of new anti-tumour reagents.

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Jan 2000
Volume 16 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Topping K, Hough V, Monson J and Greenman J: Isolation of human colorectal tumour reactive antibodies using phage display technology.. Int J Oncol 16: 187-282, 2000.
APA
Topping, K., Hough, V., Monson, J., & Greenman, J. (2000). Isolation of human colorectal tumour reactive antibodies using phage display technology.. International Journal of Oncology, 16, 187-282. https://doi.org/10.3892/ijo.16.1.187
MLA
Topping, K., Hough, V., Monson, J., Greenman, J."Isolation of human colorectal tumour reactive antibodies using phage display technology.". International Journal of Oncology 16.1 (2000): 187-282.
Chicago
Topping, K., Hough, V., Monson, J., Greenman, J."Isolation of human colorectal tumour reactive antibodies using phage display technology.". International Journal of Oncology 16, no. 1 (2000): 187-282. https://doi.org/10.3892/ijo.16.1.187