N-ethylmaleimide-enhanced phosphatidylserine externalization of human pancreatic cancer cells and immediate phosphatidylserine-mediated phagocytosis by macrophages.

  • Authors:
    • A Elnemr
    • T Ohta
    • A Yachie
    • S Fushida
    • I Ninomiya
    • G I Nishimura
    • M Yamamoto
    • S Ohkuma
    • K Miwa
  • View Affiliations

  • Published online on: June 1, 2000     https://doi.org/10.3892/ijo.16.6.1111
  • Pages: 1111-1117
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Abstract

The purpose of this study was to examine whether N-ethylmaleimide (NEM) can enhance phosphatidylserine (PS) externalization on the outer surface of cancer cells, and PS-externalized cancer cells can be phagocytosed immediately by macrophages. NEM could externalize PS of cancer cells in concentration- and time-dependent manners. When treated with 5 mM NEM for 1 h, 80% of the carcinoma cells externalized their PS. In phagocytosis assay, these cells were engulfed immediately by macrophages before undergoing apoptosis. These results suggest that NEM can immediately externalize PS of cancer cells, leading to their recognition and phagocytosis by macrophages before undergoing apoptosis morphologically.

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Jun 2000
Volume 16 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Elnemr A, Ohta T, Yachie A, Fushida S, Ninomiya I, Nishimura G, Yamamoto M, Ohkuma S and Miwa K: N-ethylmaleimide-enhanced phosphatidylserine externalization of human pancreatic cancer cells and immediate phosphatidylserine-mediated phagocytosis by macrophages.. Int J Oncol 16: 1111-1117, 2000
APA
Elnemr, A., Ohta, T., Yachie, A., Fushida, S., Ninomiya, I., Nishimura, G. ... Miwa, K. (2000). N-ethylmaleimide-enhanced phosphatidylserine externalization of human pancreatic cancer cells and immediate phosphatidylserine-mediated phagocytosis by macrophages.. International Journal of Oncology, 16, 1111-1117. https://doi.org/10.3892/ijo.16.6.1111
MLA
Elnemr, A., Ohta, T., Yachie, A., Fushida, S., Ninomiya, I., Nishimura, G., Yamamoto, M., Ohkuma, S., Miwa, K."N-ethylmaleimide-enhanced phosphatidylserine externalization of human pancreatic cancer cells and immediate phosphatidylserine-mediated phagocytosis by macrophages.". International Journal of Oncology 16.6 (2000): 1111-1117.
Chicago
Elnemr, A., Ohta, T., Yachie, A., Fushida, S., Ninomiya, I., Nishimura, G., Yamamoto, M., Ohkuma, S., Miwa, K."N-ethylmaleimide-enhanced phosphatidylserine externalization of human pancreatic cancer cells and immediate phosphatidylserine-mediated phagocytosis by macrophages.". International Journal of Oncology 16, no. 6 (2000): 1111-1117. https://doi.org/10.3892/ijo.16.6.1111