Binding of the growth suppressor p53 protein to the cell cycle regulator phosphatase cdc25C.

  • Authors:
    • N Rief
    • H Herges
    • A Prowald
    • C Götz
    • M Montenarh
  • View Affiliations

  • Published online on: July 1, 2000     https://doi.org/10.3892/ijo.17.1.189
  • Pages: 189-284
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Abstract

Human p53 is a growth suppressor which not only functions in mammalian cells but also in fission yeast. It was previously shown that the cell cycle regulating phosphatase cdc25C suppresses the p53 induced growth arrest in fission yeast. In the present study we analysed the mechanism of this suppression. We found that cdc25C directly interacts with p53. By using different deletion mutants the binding region was narrowed down on the polypeptide chain of p53 to amino acids 287-340. To test the functional significance we analysed the effect of this interaction on the DNA binding activity of p53. As shown by band shift experiments binding of cdc25C to p53 does not modify the DNA binding activity of p53. Our data suggest that the observed suppression of the p53 induced growth arrest by cdc25C might be achieved by direct binding of cdc25C to the C-terminus of p53.

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Jul 2000
Volume 17 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Rief N, Herges H, Prowald A, Götz C and Montenarh M: Binding of the growth suppressor p53 protein to the cell cycle regulator phosphatase cdc25C.. Int J Oncol 17: 189-284, 2000
APA
Rief, N., Herges, H., Prowald, A., Götz, C., & Montenarh, M. (2000). Binding of the growth suppressor p53 protein to the cell cycle regulator phosphatase cdc25C.. International Journal of Oncology, 17, 189-284. https://doi.org/10.3892/ijo.17.1.189
MLA
Rief, N., Herges, H., Prowald, A., Götz, C., Montenarh, M."Binding of the growth suppressor p53 protein to the cell cycle regulator phosphatase cdc25C.". International Journal of Oncology 17.1 (2000): 189-284.
Chicago
Rief, N., Herges, H., Prowald, A., Götz, C., Montenarh, M."Binding of the growth suppressor p53 protein to the cell cycle regulator phosphatase cdc25C.". International Journal of Oncology 17, no. 1 (2000): 189-284. https://doi.org/10.3892/ijo.17.1.189