Binding of the growth suppressor p53 protein to the cell cycle regulator phosphatase cdc25C.

Rief,N; Herges,H; Prowald,A; Götz,C; Montenarh,M

doi:10.3892/ijo.17.1.189

Jul 2000 Volume 17 Issue 1

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Jul 2000 Volume 17 Issue 1

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Article

Binding of the growth suppressor p53 protein to the cell cycle regulator phosphatase cdc25C.

Authors:
- N Rief
- H Herges
- A Prowald
- C Götz
- M Montenarh
View Affiliations / Copyright

Affiliations: Medical Biochemistry and Molecular Biology, University of the Saarland, D-66421 Homburg, Germany.
Pages: 189-284
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Published online on: July 1, 2000

https://doi.org/10.3892/ijo.17.1.189
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Abstract

Human p53 is a growth suppressor which not only functions in mammalian cells but also in fission yeast. It was previously shown that the cell cycle regulating phosphatase cdc25C suppresses the p53 induced growth arrest in fission yeast. In the present study we analysed the mechanism of this suppression. We found that cdc25C directly interacts with p53. By using different deletion mutants the binding region was narrowed down on the polypeptide chain of p53 to amino acids 287-340. To test the functional significance we analysed the effect of this interaction on the DNA binding activity of p53. As shown by band shift experiments binding of cdc25C to p53 does not modify the DNA binding activity of p53. Our data suggest that the observed suppression of the p53 induced growth arrest by cdc25C might be achieved by direct binding of cdc25C to the C-terminus of p53.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Binding of the growth suppressor p53 protein to the cell cycle regulator phosphatase cdc25C.

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Abstract

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