A new human malignant peripheral nerve sheath tumour-cell line, HS-sch-2, harbouring p53 point mutation.

Sonobe,H; Takeuchi,T; Furihata,M; Taguchi,T; Kawai,A; Ohjimi,Y; Iwasaki,H; Kaneko,Y; Ohtsuki,Y

doi:10.3892/ijo.17.2.347

Aug 2000 Volume 17 Issue 2

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Aug 2000 Volume 17 Issue 2

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Article

A new human malignant peripheral nerve sheath tumour-cell line, HS-sch-2, harbouring p53 point mutation.

Authors:
- H Sonobe
- T Takeuchi
- M Furihata
- T Taguchi
- A Kawai
- Y Ohjimi
- H Iwasaki
- Y Kaneko
- Y Ohtsuki
View Affiliations / Copyright

Affiliations: Department of Pathology, Kochi Medical School, Kohasu, Okoh, Nankoku, Kochi 783-8505, Japan.
Pages: 347-399
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Published online on: August 1, 2000

https://doi.org/10.3892/ijo.17.2.347
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Abstract

Only a few human malignant peripheral nerve sheath tumour (MPNST)-cell lines have been reported, and their characteristics have not been fully established. In this study, we established a new human cell line, HS-Sch-2, from an MPNST of the ordinary type which arose in a 54-year-old woman without von Recklinghausen's disease. This cell line was characterized by chromosome analysis, immunohistochemistry, ultrastructural examination, and direct sequencing of the p53 gene. The HS-Sch-2 cells have grown for more than 48 months in vitro, and exhibited hypotriploid karyotypes with complex chromosome abnormalities lacking a specific pattern. Histological features of the heterotranplanted nude mouse tumours were essentially the same as those of the original MPNST, with positive reactions for S-100 protein and neuron-specific enolase but not for epithelial membrane antigen, fibronectin or CD34. Ultrastructural examination in vivo revealed intricate interdigitation of long cytoplasmic processes and basal lamina-like structures. In addition, direct sequencing of the p53 gene detected a point mutation from CGT to CAT at codon 273 in exon 8. This HS-Sch-2 cell line, which exhibits distinctive morphological characteristics of MPNST and a p53 point mutation, will be useful for biological and pathological investigations of MPNST.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

A new human malignant peripheral nerve sheath tumour-cell line, HS-sch-2, harbouring p53 point mutation.

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Abstract

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