Expression of transforming growth factor beta1 and its type II receptor in mouse colon tumors induced by azoxymethane.

  • Authors:
    • Q S Wang
    • K Guda
    • A Papanikolaou
    • M Dong
    • D W Rosenberg
  • View Affiliations

  • Published online on: September 1, 2000     https://doi.org/10.3892/ijo.17.3.551
  • Pages: 551-559
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Abstract

Alterations in transforming growth factor beta1 (TGF-beta1) and its type II receptor (TbetaR-II) have been implicated in the pathogenesis of a variety of human cancers and animal tumor models. We postulated that TGF-beta1 and TbetaR-II alterations may also be involved in mouse colon tumorigenesis induced by the chemical carcinogen, azoxymethane (AOM). In the present study, normal colon tissues and AOM-induced colon tumors from SWR/J mice were analyzed for mutational changes in the TbetaR-II gene, and the expression and localization of TGF-beta1 and TbetaR-II were examined by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemisty. Direct DNA sequencing of the coding sequence of the TbetaR-II gene revealed no mutational changes in tumors induced by AOM when compared with the sequence from normal colon tissue. However, TGF-beta1 and TbetaR-II mRNA levels in tumor samples were increased 1.8-fold (p<0.01) and 1.3-fold (p<0.01), respectively, when compared with control mouse colon tissue. The results of immunohistochemical analysis of TGF-beta1 and TbetaR-II were correlated with mRNA expression data. An increase in staining intensity of both TGF-beta and TbetaR-II were observed in colon tumors. These findings suggest that alterations in the expression of TGF-beta1 and TbetaR-II may be involved in the pathogenesis of colon tumors induced by AOM in mice.

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Sep 2000
Volume 17 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Wang Q, Guda K, Papanikolaou A, Dong M and Rosenberg D: Expression of transforming growth factor beta1 and its type II receptor in mouse colon tumors induced by azoxymethane.. Int J Oncol 17: 551-559, 2000
APA
Wang, Q., Guda, K., Papanikolaou, A., Dong, M., & Rosenberg, D. (2000). Expression of transforming growth factor beta1 and its type II receptor in mouse colon tumors induced by azoxymethane.. International Journal of Oncology, 17, 551-559. https://doi.org/10.3892/ijo.17.3.551
MLA
Wang, Q., Guda, K., Papanikolaou, A., Dong, M., Rosenberg, D."Expression of transforming growth factor beta1 and its type II receptor in mouse colon tumors induced by azoxymethane.". International Journal of Oncology 17.3 (2000): 551-559.
Chicago
Wang, Q., Guda, K., Papanikolaou, A., Dong, M., Rosenberg, D."Expression of transforming growth factor beta1 and its type II receptor in mouse colon tumors induced by azoxymethane.". International Journal of Oncology 17, no. 3 (2000): 551-559. https://doi.org/10.3892/ijo.17.3.551