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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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March 2001 Volume 18 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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March 2001 Volume 18 Issue 3

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Article

Clinical application of quantitative analysis for detection of hematogenous spread of hepatocellular carcinoma by real-time PCR

  • Authors:
    • Atsushi Miyamoto
    • Hiroaki Nagano
    • Masato Sakon
    • Yoshiyuki Fujiwara
    • Yurika Sugita
    • Hidetoshi Eguchi
    • Motoi Kondo
    • Isao Arai
    • Osakuni Morimoto
    • Keizo Dono
    • Koji Umeshita
    • Shoji Nakamori
    • Morito Monden
  • View Affiliations / Copyright

    Affiliations: Department of Surgery and Clinical Oncology, Osaka University Graduate School of Medicine, Osaka, Japan
  • Pages: 527-532
    |
    Published online on: March 1, 2001
       https://doi.org/10.3892/ijo.18.3.527
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Abstract

In order to detect a hematogenous spread of tumor cells in patients with hepatocellular carcinoma, reverse transcription-polymerase chain reaction assay has been used. In this study, we quantified α-fetoprotein (AFP) messenger RNA by real-time PCR approach using LightCycler™ technique. AFP messenger RNA in the blood from 23 hepatocellular carcinoma patients undergoing hepatic resection, 31 healthy volunteers, 10 patients with liver cirrhosis and 5 patients underwent hepatectomy except for hepatocellular carcinoma was quantitated. In the real-time PCR, fluorescence was undetectable in any of the controls. On the contrary, fluorescent signals were detected in 10 out of 39 blood specimens collected from 23 HCC patients. AFP-positive status was significantly associated with the existence of multiple intrahepatic nodules. Out of 8 cases with AFP-positive status, intra- and/or extra-hepatic recurrence has been observed in 3 cases. The quantities of AFP messenger RNA in these 3 cases were relatively high among 8 cases with AFP-positive status. AFP messenger RNA was detectable by newly developed real-time PCR approach with LightCycler and it is suggested that this approach could be applicable in detection of small amounts of tumor cells in the blood of HCC patients.

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Copy and paste a formatted citation
Spandidos Publications style
Miyamoto A, Nagano H, Sakon M, Fujiwara Y, Sugita Y, Eguchi H, Kondo M, Arai I, Morimoto O, Dono K, Dono K, et al: Clinical application of quantitative analysis for detection of hematogenous spread of hepatocellular carcinoma by real-time PCR. Int J Oncol 18: 527-532, 2001.
APA
Miyamoto, A., Nagano, H., Sakon, M., Fujiwara, Y., Sugita, Y., Eguchi, H. ... Monden, M. (2001). Clinical application of quantitative analysis for detection of hematogenous spread of hepatocellular carcinoma by real-time PCR. International Journal of Oncology, 18, 527-532. https://doi.org/10.3892/ijo.18.3.527
MLA
Miyamoto, A., Nagano, H., Sakon, M., Fujiwara, Y., Sugita, Y., Eguchi, H., Kondo, M., Arai, I., Morimoto, O., Dono, K., Umeshita, K., Nakamori, S., Monden, M."Clinical application of quantitative analysis for detection of hematogenous spread of hepatocellular carcinoma by real-time PCR". International Journal of Oncology 18.3 (2001): 527-532.
Chicago
Miyamoto, A., Nagano, H., Sakon, M., Fujiwara, Y., Sugita, Y., Eguchi, H., Kondo, M., Arai, I., Morimoto, O., Dono, K., Umeshita, K., Nakamori, S., Monden, M."Clinical application of quantitative analysis for detection of hematogenous spread of hepatocellular carcinoma by real-time PCR". International Journal of Oncology 18, no. 3 (2001): 527-532. https://doi.org/10.3892/ijo.18.3.527
Copy and paste a formatted citation
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Spandidos Publications style
Miyamoto A, Nagano H, Sakon M, Fujiwara Y, Sugita Y, Eguchi H, Kondo M, Arai I, Morimoto O, Dono K, Dono K, et al: Clinical application of quantitative analysis for detection of hematogenous spread of hepatocellular carcinoma by real-time PCR. Int J Oncol 18: 527-532, 2001.
APA
Miyamoto, A., Nagano, H., Sakon, M., Fujiwara, Y., Sugita, Y., Eguchi, H. ... Monden, M. (2001). Clinical application of quantitative analysis for detection of hematogenous spread of hepatocellular carcinoma by real-time PCR. International Journal of Oncology, 18, 527-532. https://doi.org/10.3892/ijo.18.3.527
MLA
Miyamoto, A., Nagano, H., Sakon, M., Fujiwara, Y., Sugita, Y., Eguchi, H., Kondo, M., Arai, I., Morimoto, O., Dono, K., Umeshita, K., Nakamori, S., Monden, M."Clinical application of quantitative analysis for detection of hematogenous spread of hepatocellular carcinoma by real-time PCR". International Journal of Oncology 18.3 (2001): 527-532.
Chicago
Miyamoto, A., Nagano, H., Sakon, M., Fujiwara, Y., Sugita, Y., Eguchi, H., Kondo, M., Arai, I., Morimoto, O., Dono, K., Umeshita, K., Nakamori, S., Monden, M."Clinical application of quantitative analysis for detection of hematogenous spread of hepatocellular carcinoma by real-time PCR". International Journal of Oncology 18, no. 3 (2001): 527-532. https://doi.org/10.3892/ijo.18.3.527
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