Selective growth inhibition by sangivamycin of human umbilical vein endothelial cells

  • Authors:
    • Osamu Ohno
    • Yutaka Shima
    • Yoko Ikeda
    • S.-I. Kondo
    • Kuniki Kato
    • Masakazu Toi
    • Kazuo Umezawa
  • View Affiliations

  • Published online on: May 1, 2001     https://doi.org/10.3892/ijo.18.5.1009
  • Pages: 1009-1015
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Abstract

In the course of our screening for selective growth inhibitors of human umbilical vein endothelial cells (HUVECs), we isolated sangivamycin from the culture filtrate of Streptomyces. It inhibited the growth of HUVECs at ≈30 times lower concentration than that needed to inhibit the growth of WI-38 human fibroblasts. Structurally-related nucleosides, such as toyocamycin, tubercidin, and formycins A and B, did not show the differential inhibition. Although sangivamycin is known to inhibit protein kinase C, other protein kinase C inhibitors did not inhibit the growth of HUVECs selectively. Sangivamycin effectively inhibited S-phase induction in HUVECs, like TNP-470 and LLnL, known selective inhibitors. However, unlike them sangivamycin did not induce p21 expression. On the other hand, sangivamycin was found to inhibit DNA synthesis selectively in HUVECs. Thus, sangivamycin was shown to be a new selective growth inhibitor of HUVECs acting on DNA synthesis.

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May 2001
Volume 18 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ohno O, Shima Y, Ikeda Y, Kondo S, Kato K, Toi M and Umezawa K: Selective growth inhibition by sangivamycin of human umbilical vein endothelial cells. Int J Oncol 18: 1009-1015, 2001
APA
Ohno, O., Shima, Y., Ikeda, Y., Kondo, S., Kato, K., Toi, M., & Umezawa, K. (2001). Selective growth inhibition by sangivamycin of human umbilical vein endothelial cells. International Journal of Oncology, 18, 1009-1015. https://doi.org/10.3892/ijo.18.5.1009
MLA
Ohno, O., Shima, Y., Ikeda, Y., Kondo, S., Kato, K., Toi, M., Umezawa, K."Selective growth inhibition by sangivamycin of human umbilical vein endothelial cells". International Journal of Oncology 18.5 (2001): 1009-1015.
Chicago
Ohno, O., Shima, Y., Ikeda, Y., Kondo, S., Kato, K., Toi, M., Umezawa, K."Selective growth inhibition by sangivamycin of human umbilical vein endothelial cells". International Journal of Oncology 18, no. 5 (2001): 1009-1015. https://doi.org/10.3892/ijo.18.5.1009