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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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May 2001 Volume 18 Issue 5

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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May 2001 Volume 18 Issue 5

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Article

Non-isotopic silver-stained SSCP is more sensitive than automated direct sequencing for the detection of PTEN mutations in a mixture of DNA extracted from normal and tumor cells

  • Authors:
    • X. Fan
    • F. B. Furnari
    • W. K. Cavenee
    • J. S. Castresana

  • View Affiliations / Copyright

    Affiliations: Department of Genetics, University of Navarre, E-31008 Pamplona, Spain
  • Pages: 1023-1026
    |
    Published online on: May 1, 2001
       https://doi.org/10.3892/ijo.18.5.1023
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Abstract

The sensitivity of non-isotopic PCR-SSCP was compared to direct sequencing of PTEN exons. DNA from leukocytes derived from healthy donors, and from the glioblastoma cell line LN319 was extracted and mixed in different proportions from 0 to 100%. The LN319 cell line contains a point mutation at codon 15 exon 1 of the PTEN gene. The PCR-SSCP experiments demonstrated mutations in samples containing as little as 10% tumor DNA. In contrast, direct DNA sequencing experiments were less sensitive, requiring 30-70% of tumor DNA in the sample, depending on the DNA strand sequenced. In conclusion, PCR-SSCP, in our hands, is more sensitive than automated sequencing for detecting PTEN point mutations. We recommend to always sequence both strands, and take into account that samples containing less than 30% tumor cells should not only be sequenced, but also studied by PCR-SSCP in order to discriminate false negative results.

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Copy and paste a formatted citation
Spandidos Publications style
Fan X, Furnari FB, Cavenee WK and Castresana JS: Non-isotopic silver-stained SSCP is more sensitive than automated direct sequencing for the detection of PTEN mutations in a mixture of DNA extracted from normal and tumor cells. Int J Oncol 18: 1023-1026, 2001.
APA
Fan, X., Furnari, F.B., Cavenee, W.K., & Castresana, J.S. (2001). Non-isotopic silver-stained SSCP is more sensitive than automated direct sequencing for the detection of PTEN mutations in a mixture of DNA extracted from normal and tumor cells. International Journal of Oncology, 18, 1023-1026. https://doi.org/10.3892/ijo.18.5.1023
MLA
Fan, X., Furnari, F. B., Cavenee, W. K., Castresana, J. S."Non-isotopic silver-stained SSCP is more sensitive than automated direct sequencing for the detection of PTEN mutations in a mixture of DNA extracted from normal and tumor cells". International Journal of Oncology 18.5 (2001): 1023-1026.
Chicago
Fan, X., Furnari, F. B., Cavenee, W. K., Castresana, J. S."Non-isotopic silver-stained SSCP is more sensitive than automated direct sequencing for the detection of PTEN mutations in a mixture of DNA extracted from normal and tumor cells". International Journal of Oncology 18, no. 5 (2001): 1023-1026. https://doi.org/10.3892/ijo.18.5.1023
Copy and paste a formatted citation
x
Spandidos Publications style
Fan X, Furnari FB, Cavenee WK and Castresana JS: Non-isotopic silver-stained SSCP is more sensitive than automated direct sequencing for the detection of PTEN mutations in a mixture of DNA extracted from normal and tumor cells. Int J Oncol 18: 1023-1026, 2001.
APA
Fan, X., Furnari, F.B., Cavenee, W.K., & Castresana, J.S. (2001). Non-isotopic silver-stained SSCP is more sensitive than automated direct sequencing for the detection of PTEN mutations in a mixture of DNA extracted from normal and tumor cells. International Journal of Oncology, 18, 1023-1026. https://doi.org/10.3892/ijo.18.5.1023
MLA
Fan, X., Furnari, F. B., Cavenee, W. K., Castresana, J. S."Non-isotopic silver-stained SSCP is more sensitive than automated direct sequencing for the detection of PTEN mutations in a mixture of DNA extracted from normal and tumor cells". International Journal of Oncology 18.5 (2001): 1023-1026.
Chicago
Fan, X., Furnari, F. B., Cavenee, W. K., Castresana, J. S."Non-isotopic silver-stained SSCP is more sensitive than automated direct sequencing for the detection of PTEN mutations in a mixture of DNA extracted from normal and tumor cells". International Journal of Oncology 18, no. 5 (2001): 1023-1026. https://doi.org/10.3892/ijo.18.5.1023
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