Characterization of CD44 splicing patterns in normal keratinocytes, dysplastic and squamous carcinoma cell lines

  • Authors:
    • Balvinder K. Bloor
    • Mohsen Jelvagharan
    • Kenneth N. White
    • Edward W. Odell
  • View Affiliations

  • Published online on: May 1, 2001     https://doi.org/10.3892/ijo.18.5.1053
  • Pages: 1053-1059
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Abstract

The CD44 glycoprotein is spliced from a complex gene of 10 constitutive and 10 variant exons. In this study, CD44 splicing patterns and intron 9 retention were investigated by exon-specific RT-PCR for variant exons v1-v10 and intron 9 in normal, immortalized, dysplastic and malignant keratinocytes. Expression of product was determined immunohistochemically for some of the exons. Normal keratinocytes showed one major transcript including exons v2-v10 and 3 minor transcripts. No lines showed a normal CD44 splicing pattern but rather a variety of truncated transcripts of contiguous variant exons which overall correlated with expression. Squamous cell carcinoma (SCC)-4 and SCC-9 lines showed relatively normal transcripts although protein was expressed only by SCC-9. SCC-12B2, SCC-15, SCC-25 and SCC-27 showed a series of shorter overlapping transcripts, with loss of exons v8-v10 in the major transcripts. Intron 9 was not retained in normal keratinocytes or cell lines. Despite the fact that keratinocytes constitutively express all variant exons, splicing patterns are distinctly abnormal and merit investigation as potential markers for epidermal and oral squamous malignancy.

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May 2001
Volume 18 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Bloor BK, Jelvagharan M, White KN and Odell EW: Characterization of CD44 splicing patterns in normal keratinocytes, dysplastic and squamous carcinoma cell lines. Int J Oncol 18: 1053-1059, 2001
APA
Bloor, B.K., Jelvagharan, M., White, K.N., & Odell, E.W. (2001). Characterization of CD44 splicing patterns in normal keratinocytes, dysplastic and squamous carcinoma cell lines. International Journal of Oncology, 18, 1053-1059. https://doi.org/10.3892/ijo.18.5.1053
MLA
Bloor, B. K., Jelvagharan, M., White, K. N., Odell, E. W."Characterization of CD44 splicing patterns in normal keratinocytes, dysplastic and squamous carcinoma cell lines". International Journal of Oncology 18.5 (2001): 1053-1059.
Chicago
Bloor, B. K., Jelvagharan, M., White, K. N., Odell, E. W."Characterization of CD44 splicing patterns in normal keratinocytes, dysplastic and squamous carcinoma cell lines". International Journal of Oncology 18, no. 5 (2001): 1053-1059. https://doi.org/10.3892/ijo.18.5.1053