Bcl-2 modulates Fas-mediated apoptosis in human renal cell carcinoma cell lines

  • Authors:
    • I. Hara
    • S. Hara
    • H. Miyake
    • S. Arakawa
    • S. Kamidono
  • View Affiliations

  • Published online on: June 1, 2001     https://doi.org/10.3892/ijo.18.6.1181
  • Pages: 1181-1185
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Abstract

The objective of this study was to characterize the role of Bcl-2 expression in Fas-mediated apoptosis in human renal cell carcinoma (RCC) cell lines. RT-PCR analyses showed that 10 different RCC cell lines expressed Fas, but not Fas ligand. Seven of 10 cell lines expressed Fas strongly, while 3 cell lines weakly expressed Fas by flow cytometric analyses. Measurement of the LDH concentration in the culture supernatant revealed that 6 of the 7 cell lines which expressed Fas strongly were sensitive to treatment with an agonistic anti-Fas monoclonal antibody (CH11), whereas all cell lines which weakly expressed Fas did not show Fas-mediated cell death. Furthermore, Bcl-2 expression was found only in three cell lines which were all susceptible to CH11, and the downregulation of Bcl-2 protein by treatment with antisense oligodeoxynucleotide targeting Bcl-2 gene resulted in an enhancement of cell death induced by CH11. These findings suggest that strong Fas expression is necessary for Fas-mediated cell death in RCC cell lines, and Bcl-2 has a protective role against treatment with an anti-Fas monoclonal antibody, despite the fact that Bcl-2 expression was observed only in sensitive cell lines to Fas-mediated cell death.

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June 2001
Volume 18 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Hara I, Hara S, Miyake H, Arakawa S and Kamidono S: Bcl-2 modulates Fas-mediated apoptosis in human renal cell carcinoma cell lines. Int J Oncol 18: 1181-1185, 2001
APA
Hara, I., Hara, S., Miyake, H., Arakawa, S., & Kamidono, S. (2001). Bcl-2 modulates Fas-mediated apoptosis in human renal cell carcinoma cell lines. International Journal of Oncology, 18, 1181-1185. https://doi.org/10.3892/ijo.18.6.1181
MLA
Hara, I., Hara, S., Miyake, H., Arakawa, S., Kamidono, S."Bcl-2 modulates Fas-mediated apoptosis in human renal cell carcinoma cell lines". International Journal of Oncology 18.6 (2001): 1181-1185.
Chicago
Hara, I., Hara, S., Miyake, H., Arakawa, S., Kamidono, S."Bcl-2 modulates Fas-mediated apoptosis in human renal cell carcinoma cell lines". International Journal of Oncology 18, no. 6 (2001): 1181-1185. https://doi.org/10.3892/ijo.18.6.1181