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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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July 2001 Volume 19 Issue 1

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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July 2001 Volume 19 Issue 1

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Article

Molecular cloning and characterization of human Frizzled-5 gene on chromosome 2q33.3-q34 region

  • Authors:
    • Tetsuroh Saitoh
    • Momoki Hirai
    • Masaru Katoh
  • View Affiliations / Copyright

    Affiliations: Genetics and Cell Biology Section, Genetics Division, National Cancer Center Research Institute, Tokyo 104-0045, Japan
  • Pages: 105-110
    |
    Published online on: July 1, 2001
       https://doi.org/10.3892/ijo.19.1.105
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Abstract

Hfz5 is a potent cancer associated gene, encoding WNT receptor with the potential to activate β-catenin - TCF signaling pathway. Here, human Frizzled-5 (FZD5) gene and cDNAs were cloned and characterized. FZD5 was almost identical to Hfz5, except for six amino-acid substitutions at codon 88, 262, 263, 345, 357, and 402. HF5S1 probe (nucleotide position 2036-2535 of FZD5 cDNA) hybridized to 7.5- and 3.5-kb FZD5 mRNAs, and HF5S2 probe (nucleotide position 5572-6194 of FZD5 cDNA) hybridized only to 7.5-kb FZD5 mRNA. FZD5 cDNA was polyadenylated at the nucleotide position 6534, while several FZD5 ESTs were polyadenylated at the nucleotide position 2561. The 7.5- and 3.5-kb FZD5 mRNAs were transcribed probably due to alternative splicing. FZD5 was highly expressed in fetal liver and adult pancreas, and moderately expressed in fetal lung, kidney and adult liver. Among human cancer cell lines, FZD5 was highly expressed in K-562 cells derived from chronic myelogenous leukemia. FZD5 gene, consisting of two exons, was mapped to human chromosome 2q33.3-q34 region, near the FZD7 gene and the FRA2I fragile site. These results suggest that FZD5 up-regulation might play key roles in chronic myelogenous leukemia through activation of the WNT - β-catenin - TCF signaling pathway.

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Copy and paste a formatted citation
Spandidos Publications style
Saitoh T, Hirai M and Katoh M: Molecular cloning and characterization of human Frizzled-5 gene on chromosome 2q33.3-q34 region. Int J Oncol 19: 105-110, 2001.
APA
Saitoh, T., Hirai, M., & Katoh, M. (2001). Molecular cloning and characterization of human Frizzled-5 gene on chromosome 2q33.3-q34 region. International Journal of Oncology, 19, 105-110. https://doi.org/10.3892/ijo.19.1.105
MLA
Saitoh, T., Hirai, M., Katoh, M."Molecular cloning and characterization of human Frizzled-5 gene on chromosome 2q33.3-q34 region". International Journal of Oncology 19.1 (2001): 105-110.
Chicago
Saitoh, T., Hirai, M., Katoh, M."Molecular cloning and characterization of human Frizzled-5 gene on chromosome 2q33.3-q34 region". International Journal of Oncology 19, no. 1 (2001): 105-110. https://doi.org/10.3892/ijo.19.1.105
Copy and paste a formatted citation
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Spandidos Publications style
Saitoh T, Hirai M and Katoh M: Molecular cloning and characterization of human Frizzled-5 gene on chromosome 2q33.3-q34 region. Int J Oncol 19: 105-110, 2001.
APA
Saitoh, T., Hirai, M., & Katoh, M. (2001). Molecular cloning and characterization of human Frizzled-5 gene on chromosome 2q33.3-q34 region. International Journal of Oncology, 19, 105-110. https://doi.org/10.3892/ijo.19.1.105
MLA
Saitoh, T., Hirai, M., Katoh, M."Molecular cloning and characterization of human Frizzled-5 gene on chromosome 2q33.3-q34 region". International Journal of Oncology 19.1 (2001): 105-110.
Chicago
Saitoh, T., Hirai, M., Katoh, M."Molecular cloning and characterization of human Frizzled-5 gene on chromosome 2q33.3-q34 region". International Journal of Oncology 19, no. 1 (2001): 105-110. https://doi.org/10.3892/ijo.19.1.105
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