Molecular cloning and characterization of human WNT8A

  • Authors:
    • Tetsuroh Saitoh
    • Masaru Katoh
  • View Affiliations

  • Published online on: July 1, 2001     https://doi.org/10.3892/ijo.19.1.123
  • Pages: 123-127
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Abstract

WNT - β-catenin - TCF pathway is involved in carcinogenesis and fetal development. Xenopus wnt-8 is one of the most potent Wnts with the capacity to activate β-catenin - TCF pathway in the Xenopus axis duplication assay. Here, we have cloned and characterized WNT8A, a novel human homologue of Xenopus wnt-8. The WNT8A gene, consisting of at least 6 exons, was found to encode a 351-amino-acid polypeptide with the N-terminal signal peptide, three N-linked glycosylation sites, and conserved amino-acid residues of the WNT family. WNT8A showed 63.2% total-amino-acid identity to WNT8B. C-terminal region of WNT8A, WNT8B, WNT2, WNT2B1 and WNT2B2 were longer than that of other WNTs. Among various normal human tissues and 34 human cancer cells lines, the 3.5-kb WNT8A mRNA was detected only in a human teratocarcinoma cell line NT2. These results strongly suggest that WNT8A might be implicated in development of early embryos as well as germ cell tumors through activation of the WNT - β-catenin - TCF pathway.

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July 2001
Volume 19 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Saitoh, T., & Saitoh, T. (2001). Molecular cloning and characterization of human WNT8A. International Journal of Oncology, 19, 123-127. https://doi.org/10.3892/ijo.19.1.123
MLA
Saitoh, T., Katoh, M."Molecular cloning and characterization of human WNT8A". International Journal of Oncology 19.1 (2001): 123-127.
Chicago
Saitoh, T., Katoh, M."Molecular cloning and characterization of human WNT8A". International Journal of Oncology 19, no. 1 (2001): 123-127. https://doi.org/10.3892/ijo.19.1.123