Trans-resveratrol, a grapevine-derived polyphenol, blocks hepatocyte growth factor-induced invasion of hepatocellular carcinoma cells

  • Authors:
    • Victor De Ledinghen
    • Arnaud Monvoisin
    • Veronique Neaud
    • Stephanie Krisa
    • Bernard Payrastre
    • Christiane Bedin
    • Alexis Desmouliere
    • Paulette Bioulac-Sage
    • Jean Rosenbaum
  • View Affiliations

  • Published online on: July 1, 2001     https://doi.org/10.3892/ijo.19.1.83
  • Pages: 83-88
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Abstract

We have shown that liver myofibroblasts stimulate in vitro invasion of hepatocellular carcinoma cell lines through a hepatocyte growth factor/urokinase-dependent mechanism. Resveratrol, a grapevine-derived polyphenol, has been shown to inhibit cellular events associated with tumor initiation, promotion and progression. The aim of this study was to evaluate the effects of trans-resveratrol on invasion of the human hepatoma cell line HepG2. Cell invasion was assessed using a Boyden chamber assay. Activation of the HGF signal transduction pathways was evaluated by Western blot with phospho-specific antibodies. Urokinase expression was measured by RT-PCR and zymography. Trans-resveratrol decreased hepatocyte growth factor-induced cell scattering and invasion. It also decreased cell proliferation without evidence for cytotoxicity or apoptosis. Trans-resveratrol did not decrease the level of the hepatocyte growth factor receptor c-met and did not impede the hepatocyte growth factor-induced increase in c-met precursor synthesis. Moreover, trans-resveratrol did not decrease hepatocyte growth factor-induced c-met autophosphorylation, or Akt-1 or extracellular-regulated kinases-1 and -2 activation. Finally, it did not decrease urokinase expression and did not block the catalytic activity of urokinase. In conclusion, our results demonstrate that trans-resveratrol decreases hepatocyte growth factor-induced HepG2 cell invasion by an as yet unidentified post-receptor mechanism.

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July 2001
Volume 19 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
De Ledinghen V, Monvoisin A, Neaud V, Krisa S, Payrastre B, Bedin C, Desmouliere A, Bioulac-Sage P and Rosenbaum J: Trans-resveratrol, a grapevine-derived polyphenol, blocks hepatocyte growth factor-induced invasion of hepatocellular carcinoma cells. Int J Oncol 19: 83-88, 2001
APA
De Ledinghen, V., Monvoisin, A., Neaud, V., Krisa, S., Payrastre, B., Bedin, C. ... Rosenbaum, J. (2001). Trans-resveratrol, a grapevine-derived polyphenol, blocks hepatocyte growth factor-induced invasion of hepatocellular carcinoma cells. International Journal of Oncology, 19, 83-88. https://doi.org/10.3892/ijo.19.1.83
MLA
De Ledinghen, V., Monvoisin, A., Neaud, V., Krisa, S., Payrastre, B., Bedin, C., Desmouliere, A., Bioulac-Sage, P., Rosenbaum, J."Trans-resveratrol, a grapevine-derived polyphenol, blocks hepatocyte growth factor-induced invasion of hepatocellular carcinoma cells". International Journal of Oncology 19.1 (2001): 83-88.
Chicago
De Ledinghen, V., Monvoisin, A., Neaud, V., Krisa, S., Payrastre, B., Bedin, C., Desmouliere, A., Bioulac-Sage, P., Rosenbaum, J."Trans-resveratrol, a grapevine-derived polyphenol, blocks hepatocyte growth factor-induced invasion of hepatocellular carcinoma cells". International Journal of Oncology 19, no. 1 (2001): 83-88. https://doi.org/10.3892/ijo.19.1.83