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Immortalization of human esophageal keratinocytes by E6 and E7 of human papillomavirus type 16

  • Authors:
    • Hiroshi Sashiyama
    • Yuji Shino
    • Yasutaka Kawamata
    • Yoshimi Tomita
    • Naomi Ogawa
    • Hideaki Shimada
    • Susumu Kobayashi
    • Takehide Asano
    • Takenori Ochiai
    • Hiroshi Shirasawa

  • View Affiliations

  • Published online on: July 1, 2001     https://doi.org/10.3892/ijo.19.1.97
  • Pages: 97-103
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Abstract

Transduction of human papillomavirus type 16 (HPV16) E6/E7 into primary culture of human esophageal keratinocytes using a recombinant adenovirus prolonged the life-span, while untreated cells senesced within 14-16 population doublings (PDLs). Up-regulation of telomerase activity and acquisition of serum-resistant growth were observed in the esophageal keratinocytes with extended life-span between 50 and 100 PDLs, and drastically increased after 100 PDLs. A keratinocyte sample with a polymorphism of Pro/Pro at codon 72 of p53 showed resistance to HPV16 E6/E7-induced life-span-extension and immortalization, in contrast to others with p53 polymorphisms of Arg/Arg or Arg/Pro, which did not. The high efficiency of E6/E7-induction by adenovirus vector also revealed the M1 and M2 stages of keratinocyte immortalization first described in this report.

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Journal Cover

July 2001
Volume 19 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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  • Journal Metrics
  • Impact Factor: 5.2
  • Ranked Oncology
    (total number of cites)
  • CiteScore: 11.4
  • CiteScore Rank: Q1: #40/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 1.125
  • SCImago Journal Rank (SJR): 1.105
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Spandidos Publications style
Sashiyama H, Shino Y, Kawamata Y, Tomita Y, Ogawa N, Shimada H, Kobayashi S, Asano T, Ochiai T, Shirasawa H, Shirasawa H, et al: Immortalization of human esophageal keratinocytes by E6 and E7 of human papillomavirus type 16. Int J Oncol 19: 97-103, 2001
APA
Sashiyama, H., Shino, Y., Kawamata, Y., Tomita, Y., Ogawa, N., Shimada, H. ... Shirasawa, H. (2001). Immortalization of human esophageal keratinocytes by E6 and E7 of human papillomavirus type 16. International Journal of Oncology, 19, 97-103. https://doi.org/10.3892/ijo.19.1.97
MLA
Sashiyama, H., Shino, Y., Kawamata, Y., Tomita, Y., Ogawa, N., Shimada, H., Kobayashi, S., Asano, T., Ochiai, T., Shirasawa, H."Immortalization of human esophageal keratinocytes by E6 and E7 of human papillomavirus type 16". International Journal of Oncology 19.1 (2001): 97-103.
Chicago
Sashiyama, H., Shino, Y., Kawamata, Y., Tomita, Y., Ogawa, N., Shimada, H., Kobayashi, S., Asano, T., Ochiai, T., Shirasawa, H."Immortalization of human esophageal keratinocytes by E6 and E7 of human papillomavirus type 16". International Journal of Oncology 19, no. 1 (2001): 97-103. https://doi.org/10.3892/ijo.19.1.97