Fluorescence in situ hybridization to assess transitional changes of aneuploidy for chromosomes 7, 8, 10, 12, 16, X and Y in metastatic prostate cancer following anti-androgen therapy

  • Authors:
    • Kotaro Kasahara
    • Takahiro Taguchi
    • Ichiro Yamasaki
    • Takashi Karashima
    • Masayuki Kamada
    • Kazunari Yuri
    • Taro Shuin
  • View Affiliations

  • Published online on: September 1, 2001     https://doi.org/10.3892/ijo.19.3.543
  • Pages: 543-549
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Abstract

There have been few detailed studies conducted on the cell population in relation to cytogenetic changes between the pre- and post-treatment periods in patients with prostate cancer. We investigated numerical chromosome changes associated with anti-androgen therapy, using fluorescence in situ hybridization (FISH). FISH using chromosome-specific centromeric probes was used to assess transitional changes in the frequency of aneuploidy for chromosomes 7, 8, 10, 12, 16, X, and Y in prostate cancer during the pre- and post-treatment periods. Gains of chromosomes 7, 8 and 12 were notable in the pre-treatment samples (8 out of 9 cases in chromosome 7; 8 out of 9 cases in chromosome 8; 7 out of 9 cases in chromosome 12), while a notable reduction in the number of cells with extra copies of these chromosomes was observed in post-treatment specimens. Other chromosomes did not show noticeable change in their FISH signals at each phase of clinical treatment in all 9 cases. Changes in cell number with high ploidies of chromosome 7, 8 and 12 reflect the clinical effects of anti-androgen therapy at the early phase, which might explain the androgen dependency of metastatic prostate cancer cells.

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September 2001
Volume 19 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kasahara K, Taguchi T, Yamasaki I, Karashima T, Kamada M, Yuri K and Shuin T: Fluorescence in situ hybridization to assess transitional changes of aneuploidy for chromosomes 7, 8, 10, 12, 16, X and Y in metastatic prostate cancer following anti-androgen therapy. Int J Oncol 19: 543-549, 2001.
APA
Kasahara, K., Taguchi, T., Yamasaki, I., Karashima, T., Kamada, M., Yuri, K., & Shuin, T. (2001). Fluorescence in situ hybridization to assess transitional changes of aneuploidy for chromosomes 7, 8, 10, 12, 16, X and Y in metastatic prostate cancer following anti-androgen therapy. International Journal of Oncology, 19, 543-549. https://doi.org/10.3892/ijo.19.3.543
MLA
Kasahara, K., Taguchi, T., Yamasaki, I., Karashima, T., Kamada, M., Yuri, K., Shuin, T."Fluorescence in situ hybridization to assess transitional changes of aneuploidy for chromosomes 7, 8, 10, 12, 16, X and Y in metastatic prostate cancer following anti-androgen therapy". International Journal of Oncology 19.3 (2001): 543-549.
Chicago
Kasahara, K., Taguchi, T., Yamasaki, I., Karashima, T., Kamada, M., Yuri, K., Shuin, T."Fluorescence in situ hybridization to assess transitional changes of aneuploidy for chromosomes 7, 8, 10, 12, 16, X and Y in metastatic prostate cancer following anti-androgen therapy". International Journal of Oncology 19, no. 3 (2001): 543-549. https://doi.org/10.3892/ijo.19.3.543