Enhanced GM3 expression, associated with decreased invasiveness, is induced by brefeldin A in bladder cancer cells

  • Authors:
    • Makoto Satoh
    • Akihiro Ito
    • Hisao Nojiri
    • Kazuko Handa
    • Kenji Numahata
    • Chikara Ohyama
    • Seiichi Saito
    • Senji Hoshi
    • Sen-Itiroh Hakomori
  • View Affiliations

  • Published online on: October 1, 2001     https://doi.org/10.3892/ijo.19.4.723
  • Pages: 723-731
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Abstract

We reported previously that non-invasive bladder cancer expresses high level of GM3 ganglioside, whereas invasive tumors have low levels. Since glycosphingolipid synthesis in Golgi is modified greatly by a macrocyclic lactone isolated from fungi, brefeldin A (BFA), we studied effects of BFA on expression of glycosphingolipids and on invasiveness of bladder cancer cell lines. Only GM3 synthesis in invasive tumors was greatly enhanced upon treatment with BFA; synthesis of other glycosphingolipids with lacto-series type 2 or globo-series structure in both invasive and non-invasive tumors was not changed. Invasiveness of bladder cancer cells was greatly decreased in association with the great increase of GM3 synthesis induced by BFA treatment. Level of sialyl-Lex expressed in invasive cell line YTS1, which provides the adhesive property of the cells to E-selectin, was unchanged upon BFA treatment. All the bladder cancer cell lines, regardless of invasiveness, highly express tetraspanin CD9. GM3 has been implicated as a co-factor of CD9 in control of tumor cell motility. Down-regulation of CD9 is associated with metastatic properties of tumor cells and survival of patients with colonic cancer. Therefore, enhanced synthesis of GM3 induced by BFA, causing decrease of invasiveness in bladder cancer, is ascribable to the capability of GM3 to interconnect integrin with CD9, in analogy to colonic cancer and perhaps many other types of cancer.

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October 2001
Volume 19 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Satoh M, Ito A, Nojiri H, Handa K, Numahata K, Ohyama C, Saito S, Hoshi S and Hakomori S: Enhanced GM3 expression, associated with decreased invasiveness, is induced by brefeldin A in bladder cancer cells. Int J Oncol 19: 723-731, 2001.
APA
Satoh, M., Ito, A., Nojiri, H., Handa, K., Numahata, K., Ohyama, C. ... Hakomori, S. (2001). Enhanced GM3 expression, associated with decreased invasiveness, is induced by brefeldin A in bladder cancer cells. International Journal of Oncology, 19, 723-731. https://doi.org/10.3892/ijo.19.4.723
MLA
Satoh, M., Ito, A., Nojiri, H., Handa, K., Numahata, K., Ohyama, C., Saito, S., Hoshi, S., Hakomori, S."Enhanced GM3 expression, associated with decreased invasiveness, is induced by brefeldin A in bladder cancer cells". International Journal of Oncology 19.4 (2001): 723-731.
Chicago
Satoh, M., Ito, A., Nojiri, H., Handa, K., Numahata, K., Ohyama, C., Saito, S., Hoshi, S., Hakomori, S."Enhanced GM3 expression, associated with decreased invasiveness, is induced by brefeldin A in bladder cancer cells". International Journal of Oncology 19, no. 4 (2001): 723-731. https://doi.org/10.3892/ijo.19.4.723