FAS-associated factor 1 interacts with protein kinase CK2 in vivo upon apoptosis induction

  • Authors:
    • B. Guerra
    • B. Boldyreff
    • Olaf-Georg Issinger
  • View Affiliations

  • Published online on: December 1, 2001     https://doi.org/10.3892/ijo.19.6.1117
  • Pages: 1117-1126
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Abstract

We show here that in several different cell lines protein kinase CK2 and Fas-associated factor 1 (FAF1) exist together in a complex which is stable to high monovalent salt concentration. The CK2/FAF1 complex formation is significantly increased after induction of apoptosis with various DNA damaging agents. Interestingly this effect is only seen in cell lines with an embryonic origin and not when cells have entered a differentiated state. It is further shown that the CK2 specific phosphorylation sites in the FAF1 molecule, i.e. serines 289 and 291 influence this complex formation. Mutation of the CK2 phosphorylation sites in the FAF1 molecule to alanine leads to a 1.5 to 2.0-fold higher association between CK2 and FAF1. Since the CK2 activity did not increase concomitantly with the complex formation we conclude that the FAF1 becomes to the CK2 enzyme so that a normal enzyme catalysis does not take place anymore. Subcellular localization experiments involving CK2 subunits and FAF1 show a co-localization of both CK2 subunits and FAF1 in the peri-nuclear cytoplasm. The majority of CK2 subunits is found in the nucleus. FAF1 is also found in the nucleoli. The results obtained further support the view that protein kinase CK2 plays an important role in certain steps of apoptosis.

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December 2001
Volume 19 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Guerra B, Boldyreff B and Issinger O: FAS-associated factor 1 interacts with protein kinase CK2 in vivo upon apoptosis induction. Int J Oncol 19: 1117-1126, 2001
APA
Guerra, B., Boldyreff, B., & Issinger, O. (2001). FAS-associated factor 1 interacts with protein kinase CK2 in vivo upon apoptosis induction. International Journal of Oncology, 19, 1117-1126. https://doi.org/10.3892/ijo.19.6.1117
MLA
Guerra, B., Boldyreff, B., Issinger, O."FAS-associated factor 1 interacts with protein kinase CK2 in vivo upon apoptosis induction". International Journal of Oncology 19.6 (2001): 1117-1126.
Chicago
Guerra, B., Boldyreff, B., Issinger, O."FAS-associated factor 1 interacts with protein kinase CK2 in vivo upon apoptosis induction". International Journal of Oncology 19, no. 6 (2001): 1117-1126. https://doi.org/10.3892/ijo.19.6.1117