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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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January 1993 Volume 2 Issue 1

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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January 1993 Volume 2 Issue 1

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Article

THE ISOLATION AND IDENTIFICATION OF CIS-DIAMMINEDICHLOROPLATINUM (II)-DNA ADDUCTS BY ANION-EXCHANGE HPLC AND INDUCTIVELY COUPLED PLASMA MASS-SPECTROMETRY

  • Authors:
    • JG MORRISON
    • D BISSETT
    • IFD STEPHENS
    • K MCKAY
    • R BROWN
    • MA GRAHAM
    • AM FICHTINGER-SCHEPMAN
    • DJ KERR
  • View Affiliations / Copyright

    Affiliations: UNIV GLASGOW, DEPT MED ONCOL,CRC,ALEXANDER STONE BLDG, GARSCUBE ESTATE, GLASGOW G61 1BD, SCOTLAND. SCOTTISH UNIV RES & REACTOR CTR, E KILBRIDE G75 0QU, LANARK, SCOTLAND. TNO, MED BIOL LAB, RIJSWIJK, NETHERLANDS. QUEEN ELIZABETH HOSP, DEPT MED ONCOL, CRC, BIRMINGHAM B15 2TH, W MIDLANDS, ENGLAND.
  • Pages: 33-37
    |
    Published online on: January 1, 1993
       https://doi.org/10.3892/ijo.2.1.33
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Abstract

The adducts produced by the reaction of cis-diamminedichloroplatinum(II) with DNA have previously been isolated and characterised. These adducts may be measured at the cellular level by immunochemical detection but the accuracy of this assay is dependent on the number of adducts per nucleotide. We have developed a novel assay for cisplatin-DNA adducts, utilising an established method in which platinated DNA is digested to form a mixture of nucleotides and adducts; these are then separated by anion exchange HPLC. The number of cisplatin-DNA adducts is determined by measurement of the platinum content of the HPLC fractions by inductively coupled plasma mass spectrometry. The assay has been validated by cochromatography of purified drug-DNA adducts whose identity has been confirmed by NMR. We describe an application of the assay, namely the measurement of in vitro removal of cisplatin-DNA adducts from calf thymus DNA by cell free extracts derived from tumour cell lines. Adduct removal is dependent on both the amount of extract protein and the duration of the reaction. Almost 70% of adducts are removed from 5mug of DNA (drug:nucleotide ratio 0.08) by 80mug of extract. Other potential applications of the assay are discussed.

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Copy and paste a formatted citation
Spandidos Publications style
MORRISON J, BISSETT D, STEPHENS I, MCKAY K, BROWN R, GRAHAM M, FICHTINGER-SCHEPMAN A and KERR D: THE ISOLATION AND IDENTIFICATION OF CIS-DIAMMINEDICHLOROPLATINUM (II)-DNA ADDUCTS BY ANION-EXCHANGE HPLC AND INDUCTIVELY COUPLED PLASMA MASS-SPECTROMETRY. Int J Oncol 2: 33-37, 1993.
APA
MORRISON, J., BISSETT, D., STEPHENS, I., MCKAY, K., BROWN, R., GRAHAM, M. ... KERR, D. (1993). THE ISOLATION AND IDENTIFICATION OF CIS-DIAMMINEDICHLOROPLATINUM (II)-DNA ADDUCTS BY ANION-EXCHANGE HPLC AND INDUCTIVELY COUPLED PLASMA MASS-SPECTROMETRY. International Journal of Oncology, 2, 33-37. https://doi.org/10.3892/ijo.2.1.33
MLA
MORRISON, J., BISSETT, D., STEPHENS, I., MCKAY, K., BROWN, R., GRAHAM, M., FICHTINGER-SCHEPMAN, A., KERR, D."THE ISOLATION AND IDENTIFICATION OF CIS-DIAMMINEDICHLOROPLATINUM (II)-DNA ADDUCTS BY ANION-EXCHANGE HPLC AND INDUCTIVELY COUPLED PLASMA MASS-SPECTROMETRY". International Journal of Oncology 2.1 (1993): 33-37.
Chicago
MORRISON, J., BISSETT, D., STEPHENS, I., MCKAY, K., BROWN, R., GRAHAM, M., FICHTINGER-SCHEPMAN, A., KERR, D."THE ISOLATION AND IDENTIFICATION OF CIS-DIAMMINEDICHLOROPLATINUM (II)-DNA ADDUCTS BY ANION-EXCHANGE HPLC AND INDUCTIVELY COUPLED PLASMA MASS-SPECTROMETRY". International Journal of Oncology 2, no. 1 (1993): 33-37. https://doi.org/10.3892/ijo.2.1.33
Copy and paste a formatted citation
x
Spandidos Publications style
MORRISON J, BISSETT D, STEPHENS I, MCKAY K, BROWN R, GRAHAM M, FICHTINGER-SCHEPMAN A and KERR D: THE ISOLATION AND IDENTIFICATION OF CIS-DIAMMINEDICHLOROPLATINUM (II)-DNA ADDUCTS BY ANION-EXCHANGE HPLC AND INDUCTIVELY COUPLED PLASMA MASS-SPECTROMETRY. Int J Oncol 2: 33-37, 1993.
APA
MORRISON, J., BISSETT, D., STEPHENS, I., MCKAY, K., BROWN, R., GRAHAM, M. ... KERR, D. (1993). THE ISOLATION AND IDENTIFICATION OF CIS-DIAMMINEDICHLOROPLATINUM (II)-DNA ADDUCTS BY ANION-EXCHANGE HPLC AND INDUCTIVELY COUPLED PLASMA MASS-SPECTROMETRY. International Journal of Oncology, 2, 33-37. https://doi.org/10.3892/ijo.2.1.33
MLA
MORRISON, J., BISSETT, D., STEPHENS, I., MCKAY, K., BROWN, R., GRAHAM, M., FICHTINGER-SCHEPMAN, A., KERR, D."THE ISOLATION AND IDENTIFICATION OF CIS-DIAMMINEDICHLOROPLATINUM (II)-DNA ADDUCTS BY ANION-EXCHANGE HPLC AND INDUCTIVELY COUPLED PLASMA MASS-SPECTROMETRY". International Journal of Oncology 2.1 (1993): 33-37.
Chicago
MORRISON, J., BISSETT, D., STEPHENS, I., MCKAY, K., BROWN, R., GRAHAM, M., FICHTINGER-SCHEPMAN, A., KERR, D."THE ISOLATION AND IDENTIFICATION OF CIS-DIAMMINEDICHLOROPLATINUM (II)-DNA ADDUCTS BY ANION-EXCHANGE HPLC AND INDUCTIVELY COUPLED PLASMA MASS-SPECTROMETRY". International Journal of Oncology 2, no. 1 (1993): 33-37. https://doi.org/10.3892/ijo.2.1.33
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