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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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January 1993 Volume 2 Issue 1

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January 1993 Volume 2 Issue 1

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Article

A NEW MICROPERFUSION SYSTEM FOR THE CULTIVATION OF TUMOR-CELLS INVITRO - APPROACH TO INTEGRATE PHARMACOKINETIC PARAMETERS IN SCREENING ASSAYS FOR CYTOSTATIC DRUGS

  • Authors:
    • S GIMMEL
    • AW KINAWI
    • HR MAURER

  • View Affiliations / Copyright

    Affiliations: FREIEN UNIV BERLIN,INST PHARM,KELCHSTR 31,W-1000 BERLIN 41,GERMANY.
  • Pages: 39-46
    |
    Published online on: January 1, 1993
       https://doi.org/10.3892/ijo.2.1.39
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Abstract

By a newly introduced microperfusion system absorption and elimination rates can be simulated in vitro. This article describes the optimization of culture conditions (medium composition, membrane filters, pumping rates, and stirring speeds) of tumor cell lines (L1210, KB) maintained in suspension in an ultrafiltration-flat chamber. Viability and colony-forming ability are measured. Our results indicate that tumor cells can be cultured under serum-free conditions over a five hour incubation period with only minimal decrease in colony-forming ability. Survival of cells is independent from the pumping rate in the tested range, but is dependent of the stirring speed. Each cell line requires its own stirring speed. Ultrafiltration membranes with minimal nonspecific adsorption properties proved to be the best in terms of cell adsorption and toxicity to retain cells in the chamber. This system might improve the tumor cell colony assay for cytostatic drug screening.

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Copy and paste a formatted citation
Spandidos Publications style
GIMMEL S, KINAWI A and MAURER H: A NEW MICROPERFUSION SYSTEM FOR THE CULTIVATION OF TUMOR-CELLS INVITRO - APPROACH TO INTEGRATE PHARMACOKINETIC PARAMETERS IN SCREENING ASSAYS FOR CYTOSTATIC DRUGS. Int J Oncol 2: 39-46, 1993.
APA
GIMMEL, S., KINAWI, A., & MAURER, H. (1993). A NEW MICROPERFUSION SYSTEM FOR THE CULTIVATION OF TUMOR-CELLS INVITRO - APPROACH TO INTEGRATE PHARMACOKINETIC PARAMETERS IN SCREENING ASSAYS FOR CYTOSTATIC DRUGS. International Journal of Oncology, 2, 39-46. https://doi.org/10.3892/ijo.2.1.39
MLA
GIMMEL, S., KINAWI, A., MAURER, H."A NEW MICROPERFUSION SYSTEM FOR THE CULTIVATION OF TUMOR-CELLS INVITRO - APPROACH TO INTEGRATE PHARMACOKINETIC PARAMETERS IN SCREENING ASSAYS FOR CYTOSTATIC DRUGS". International Journal of Oncology 2.1 (1993): 39-46.
Chicago
GIMMEL, S., KINAWI, A., MAURER, H."A NEW MICROPERFUSION SYSTEM FOR THE CULTIVATION OF TUMOR-CELLS INVITRO - APPROACH TO INTEGRATE PHARMACOKINETIC PARAMETERS IN SCREENING ASSAYS FOR CYTOSTATIC DRUGS". International Journal of Oncology 2, no. 1 (1993): 39-46. https://doi.org/10.3892/ijo.2.1.39
Copy and paste a formatted citation
x
Spandidos Publications style
GIMMEL S, KINAWI A and MAURER H: A NEW MICROPERFUSION SYSTEM FOR THE CULTIVATION OF TUMOR-CELLS INVITRO - APPROACH TO INTEGRATE PHARMACOKINETIC PARAMETERS IN SCREENING ASSAYS FOR CYTOSTATIC DRUGS. Int J Oncol 2: 39-46, 1993.
APA
GIMMEL, S., KINAWI, A., & MAURER, H. (1993). A NEW MICROPERFUSION SYSTEM FOR THE CULTIVATION OF TUMOR-CELLS INVITRO - APPROACH TO INTEGRATE PHARMACOKINETIC PARAMETERS IN SCREENING ASSAYS FOR CYTOSTATIC DRUGS. International Journal of Oncology, 2, 39-46. https://doi.org/10.3892/ijo.2.1.39
MLA
GIMMEL, S., KINAWI, A., MAURER, H."A NEW MICROPERFUSION SYSTEM FOR THE CULTIVATION OF TUMOR-CELLS INVITRO - APPROACH TO INTEGRATE PHARMACOKINETIC PARAMETERS IN SCREENING ASSAYS FOR CYTOSTATIC DRUGS". International Journal of Oncology 2.1 (1993): 39-46.
Chicago
GIMMEL, S., KINAWI, A., MAURER, H."A NEW MICROPERFUSION SYSTEM FOR THE CULTIVATION OF TUMOR-CELLS INVITRO - APPROACH TO INTEGRATE PHARMACOKINETIC PARAMETERS IN SCREENING ASSAYS FOR CYTOSTATIC DRUGS". International Journal of Oncology 2, no. 1 (1993): 39-46. https://doi.org/10.3892/ijo.2.1.39
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