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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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March 1993 Volume 2 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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March 1993 Volume 2 Issue 3

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Article

A SEMIAUTOMATIC MICROASSAY TO MEASURE HUMAN TUMOR CLONOGENIC GROWTH-INVITRO

  • Authors:
    • F GARBIN
    • K ECKERT
    • HR MAURER

  • View Affiliations / Copyright

    Affiliations: FREE UNIV BERLIN,INST PHARM,KELCHSTR 31,W-1000 BERLIN 41,GERMANY.
  • Pages: 357-361
    |
    Published online on: March 1, 1993
       https://doi.org/10.3892/ijo.2.3.357
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Abstract

A semi-automatic micromethod was developed using 96-well microtiter plates in combination with a video monitoring system to assay human tumor clonogenic growth in vitro. The conditions to follow the growth of K562 human myeloid colonies were optimized and validated: About 250 cells in a 40 mul agar mixture are to be seeded per well. The colony number is proportional to the number of seeded cells. The cloning efficiency is about 75% and correlates well with the established glass capillary method. The system precision of the automatic colony counter, expressed by the coefficient of variation, is less than 2%. The mean of the intra- and inter assay coefficient of variation is low (8.5% and 7%, respectively). The microassay was applied to measure the lymphokine-activated killer (LAK)-activity of peripheral blood mononuclear cells (PBMC) from healthy donors against K562 target cells and to examine the effects of various thymic hormone preparations on the cytotoxicity of generated LAK cells. Thus, the new microassay provides a useful tool for measuring clonogenic tumor cell growth and its modulation by different agents on many samples in a short time.

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Copy and paste a formatted citation
Spandidos Publications style
GARBIN F, ECKERT K and MAURER H: A SEMIAUTOMATIC MICROASSAY TO MEASURE HUMAN TUMOR CLONOGENIC GROWTH-INVITRO. Int J Oncol 2: 357-361, 1993.
APA
GARBIN, F., ECKERT, K., & MAURER, H. (1993). A SEMIAUTOMATIC MICROASSAY TO MEASURE HUMAN TUMOR CLONOGENIC GROWTH-INVITRO. International Journal of Oncology, 2, 357-361. https://doi.org/10.3892/ijo.2.3.357
MLA
GARBIN, F., ECKERT, K., MAURER, H."A SEMIAUTOMATIC MICROASSAY TO MEASURE HUMAN TUMOR CLONOGENIC GROWTH-INVITRO". International Journal of Oncology 2.3 (1993): 357-361.
Chicago
GARBIN, F., ECKERT, K., MAURER, H."A SEMIAUTOMATIC MICROASSAY TO MEASURE HUMAN TUMOR CLONOGENIC GROWTH-INVITRO". International Journal of Oncology 2, no. 3 (1993): 357-361. https://doi.org/10.3892/ijo.2.3.357
Copy and paste a formatted citation
x
Spandidos Publications style
GARBIN F, ECKERT K and MAURER H: A SEMIAUTOMATIC MICROASSAY TO MEASURE HUMAN TUMOR CLONOGENIC GROWTH-INVITRO. Int J Oncol 2: 357-361, 1993.
APA
GARBIN, F., ECKERT, K., & MAURER, H. (1993). A SEMIAUTOMATIC MICROASSAY TO MEASURE HUMAN TUMOR CLONOGENIC GROWTH-INVITRO. International Journal of Oncology, 2, 357-361. https://doi.org/10.3892/ijo.2.3.357
MLA
GARBIN, F., ECKERT, K., MAURER, H."A SEMIAUTOMATIC MICROASSAY TO MEASURE HUMAN TUMOR CLONOGENIC GROWTH-INVITRO". International Journal of Oncology 2.3 (1993): 357-361.
Chicago
GARBIN, F., ECKERT, K., MAURER, H."A SEMIAUTOMATIC MICROASSAY TO MEASURE HUMAN TUMOR CLONOGENIC GROWTH-INVITRO". International Journal of Oncology 2, no. 3 (1993): 357-361. https://doi.org/10.3892/ijo.2.3.357
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