Human thrombospondin 2 inhibits proliferation of microvascular endothelial cells

  • Authors:
    • Yasushi Tomii
    • Junichiro Kamochi
    • Hitoshi Yamazaki
    • Nobuko Sawa
    • Tetsuji Tokunaga
    • Yasuyuki Ohnishi
    • Hiroshi Kijima
    • Yoshito Ueyama
    • Norikazu Tamaoki
    • Masato Nakamura
  • View Affiliations

  • Published online on: February 1, 2002     https://doi.org/10.3892/ijo.20.2.339
  • Pages:339-342
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Abstract

This study was performed to characterize human thrombospondin 2 (TSP2). TSP2 has recently attracted attention as an endogenous negative regulator of angiogenesis in tumorigenesis. We cloned and transfected human TSP2 cDNA into the human colon cancer cell line SW-480. Stable transfectants (TSP2-1, TSP2-6) overexpressing TSP2 were established. Growth characteristics of TSP2-transfectants were investigated in vitro and in vivo. TSP2-transfectants showed similar growth properties to vector-transfectants and wild-type SW-480 cells. The overexpression of transfected human TSP2 cDNA did not affect proliferation of SW-480 cells. When the conditioned media of TSP2-transfectants were added to cultures of bovine pulmonary microvascular endothelial cells (BPMEC), the BPMEC proliferation was significantly inhibited. These results suggested that human TSP2 is a potential inhibitor of angiogenesis.

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February 2002
Volume 20 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

2015 Impact Factor: 3.018
Ranked #30/211 Oncology
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APA
Tomii, Y., Kamochi, J., Yamazaki, H., Sawa, N., Tokunaga, T., Ohnishi, Y. ... Nakamura, M. (2002). Human thrombospondin 2 inhibits proliferation of microvascular endothelial cells. International Journal of Oncology, 20, 339-342. https://doi.org/10.3892/ijo.20.2.339
MLA
Tomii, Y., Kamochi, J., Yamazaki, H., Sawa, N., Tokunaga, T., Ohnishi, Y., Kijima, H., Ueyama, Y., Tamaoki, N., Nakamura, M."Human thrombospondin 2 inhibits proliferation of microvascular endothelial cells". International Journal of Oncology 20.2 (2002): 339-342.
Chicago
Tomii, Y., Kamochi, J., Yamazaki, H., Sawa, N., Tokunaga, T., Ohnishi, Y., Kijima, H., Ueyama, Y., Tamaoki, N., Nakamura, M."Human thrombospondin 2 inhibits proliferation of microvascular endothelial cells". International Journal of Oncology 20, no. 2 (2002): 339-342. https://doi.org/10.3892/ijo.20.2.339