Lectin isolation and detection of N-glycoproteins bearing sialic acid and L-fucose residues in human colorectal mucosa and in adenocarcinoma biopsies

  • Authors:
    • Alejandro De Carlos
    • Amado Caride-Castro
    • Vicenta S. Martinez-Zorzano
    • Maria Paez de la Cadena
    • Francisco J. Rodriguez-Berrocal
  • View Affiliations

  • Published online on: February 1, 2002     https://doi.org/10.3892/ijo.20.2.367
  • Pages:367-372
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Abstract

A method to improve the reactivity to specific lectins of N-glycoproteins isolated from human colorectal mucosa and from adenocarcinoma biopsies was developed using a combination of techniques. Total protein extracts were subjected to affinity chromatography, using the immobilised lectin Concanavalin A coupled to Sepharose, by fast performance liquid chromatography (FPLC). N-glycoprotein enriched fractions were resolved by SDS-PAGE, transferred to PVDF membranes and incubated with various lectins. Digoxigenin-conjugated SNA I and MAA lectins were used to detect sialic acid residues. Biotin-conjugated UEA I lectin was used to detect L-fucose residues. By this method, lectin-binding N-glycoproteins were found in a broad relative molecular mass (Mr) range (from 47 to 205 kDa). No tissue-specific N-glycoproteins were observed when human colorectal mucosa and adenocarcinoma samples were compared.

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February 2002
Volume 20 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
De Carlos, A., Caride-Castro, A., Martinez-Zorzano, V.S., Paez de la Cadena, M., & Rodriguez-Berrocal, F.J. (2002). Lectin isolation and detection of N-glycoproteins bearing sialic acid and L-fucose residues in human colorectal mucosa and in adenocarcinoma biopsies. International Journal of Oncology, 20, 367-372. https://doi.org/10.3892/ijo.20.2.367
MLA
De Carlos, A., Caride-Castro, A., Martinez-Zorzano, V. S., Paez de la Cadena, M., Rodriguez-Berrocal, F. J."Lectin isolation and detection of N-glycoproteins bearing sialic acid and L-fucose residues in human colorectal mucosa and in adenocarcinoma biopsies". International Journal of Oncology 20.2 (2002): 367-372.
Chicago
De Carlos, A., Caride-Castro, A., Martinez-Zorzano, V. S., Paez de la Cadena, M., Rodriguez-Berrocal, F. J."Lectin isolation and detection of N-glycoproteins bearing sialic acid and L-fucose residues in human colorectal mucosa and in adenocarcinoma biopsies". International Journal of Oncology 20, no. 2 (2002): 367-372. https://doi.org/10.3892/ijo.20.2.367