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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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February 2002 Volume 20 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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February 2002 Volume 20 Issue 2

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Article

Lectin isolation and detection of N-glycoproteins bearing sialic acid and L-fucose residues in human colorectal mucosa and in adenocarcinoma biopsies

  • Authors:
    • Alejandro De Carlos
    • Amado Caride-Castro
    • Vicenta S. Martinez-Zorzano
    • Maria Paez de la Cadena
    • Francisco J. Rodriguez-Berrocal
  • View Affiliations / Copyright

    Affiliations: Facultad de Ciencias, Universidad de Vigo, E-36200 Vigo, Spain
  • Pages: 367-372
    |
    Published online on: February 1, 2002
       https://doi.org/10.3892/ijo.20.2.367
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Abstract

A method to improve the reactivity to specific lectins of N-glycoproteins isolated from human colorectal mucosa and from adenocarcinoma biopsies was developed using a combination of techniques. Total protein extracts were subjected to affinity chromatography, using the immobilised lectin Concanavalin A coupled to Sepharose, by fast performance liquid chromatography (FPLC). N-glycoprotein enriched fractions were resolved by SDS-PAGE, transferred to PVDF membranes and incubated with various lectins. Digoxigenin-conjugated SNA I and MAA lectins were used to detect sialic acid residues. Biotin-conjugated UEA I lectin was used to detect L-fucose residues. By this method, lectin-binding N-glycoproteins were found in a broad relative molecular mass (Mr) range (from 47 to 205 kDa). No tissue-specific N-glycoproteins were observed when human colorectal mucosa and adenocarcinoma samples were compared.

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Copy and paste a formatted citation
Spandidos Publications style
De Carlos A, Caride-Castro A, Martinez-Zorzano VS, Paez de la Cadena M and Rodriguez-Berrocal FJ: Lectin isolation and detection of N-glycoproteins bearing sialic acid and L-fucose residues in human colorectal mucosa and in adenocarcinoma biopsies. Int J Oncol 20: 367-372, 2002.
APA
De Carlos, A., Caride-Castro, A., Martinez-Zorzano, V.S., Paez de la Cadena, M., & Rodriguez-Berrocal, F.J. (2002). Lectin isolation and detection of N-glycoproteins bearing sialic acid and L-fucose residues in human colorectal mucosa and in adenocarcinoma biopsies. International Journal of Oncology, 20, 367-372. https://doi.org/10.3892/ijo.20.2.367
MLA
De Carlos, A., Caride-Castro, A., Martinez-Zorzano, V. S., Paez de la Cadena, M., Rodriguez-Berrocal, F. J."Lectin isolation and detection of N-glycoproteins bearing sialic acid and L-fucose residues in human colorectal mucosa and in adenocarcinoma biopsies". International Journal of Oncology 20.2 (2002): 367-372.
Chicago
De Carlos, A., Caride-Castro, A., Martinez-Zorzano, V. S., Paez de la Cadena, M., Rodriguez-Berrocal, F. J."Lectin isolation and detection of N-glycoproteins bearing sialic acid and L-fucose residues in human colorectal mucosa and in adenocarcinoma biopsies". International Journal of Oncology 20, no. 2 (2002): 367-372. https://doi.org/10.3892/ijo.20.2.367
Copy and paste a formatted citation
x
Spandidos Publications style
De Carlos A, Caride-Castro A, Martinez-Zorzano VS, Paez de la Cadena M and Rodriguez-Berrocal FJ: Lectin isolation and detection of N-glycoproteins bearing sialic acid and L-fucose residues in human colorectal mucosa and in adenocarcinoma biopsies. Int J Oncol 20: 367-372, 2002.
APA
De Carlos, A., Caride-Castro, A., Martinez-Zorzano, V.S., Paez de la Cadena, M., & Rodriguez-Berrocal, F.J. (2002). Lectin isolation and detection of N-glycoproteins bearing sialic acid and L-fucose residues in human colorectal mucosa and in adenocarcinoma biopsies. International Journal of Oncology, 20, 367-372. https://doi.org/10.3892/ijo.20.2.367
MLA
De Carlos, A., Caride-Castro, A., Martinez-Zorzano, V. S., Paez de la Cadena, M., Rodriguez-Berrocal, F. J."Lectin isolation and detection of N-glycoproteins bearing sialic acid and L-fucose residues in human colorectal mucosa and in adenocarcinoma biopsies". International Journal of Oncology 20.2 (2002): 367-372.
Chicago
De Carlos, A., Caride-Castro, A., Martinez-Zorzano, V. S., Paez de la Cadena, M., Rodriguez-Berrocal, F. J."Lectin isolation and detection of N-glycoproteins bearing sialic acid and L-fucose residues in human colorectal mucosa and in adenocarcinoma biopsies". International Journal of Oncology 20, no. 2 (2002): 367-372. https://doi.org/10.3892/ijo.20.2.367
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