Immunohistochemistry of cyclin D1 and β-catenin, and mutational analysis of exon 3 of β-catenin gene in parathyroid adenomas

  • Authors:
    • Satoshi Ikeda
    • Yasuyo Ishizaki
    • Yosuke Shimizu
    • Masahiko Fujimori
    • Yasutomo Ojima
    • Masazumi Okajima
    • Keizo Sugino
    • Toshimasa Asahara
  • View Affiliations

  • Published online on: March 1, 2002     https://doi.org/10.3892/ijo.20.3.463
  • Pages: 463-466
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Abstract

Parathyroid adenomas are benign uniglandular tumors and are the most common cause of primary hyperparathyroidism. Several genetic changes in parathyroid tumors, including inactivation of tumor suppressor genes, activation of oncogenes and loss of heterozygosity at several chromosomal loci, have been reported. In this study, we analyzed the status of cyclin D1 and β-catenin in 24 cases of parathyroid adenoma. Immunohistochemistry of cyclin D1 showed positive staining in 9 (37.5%) of the 24 parathyroid adenomas. The status of β-catenin, which has recently been identified as a regulator of cyclin D1 transcription, was examined by direct sequencing of exon 3 of the β-catenin gene and immunohistochemistry of β-catenin protein, but neither mutation nor accumulation of β-catenin was detected in any of the cases. These results indicate that cyclin D1 is frequently accumulated in parathyroid adenomas, independently of dysfunction in the Wnt signaling pathway.

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March 2002
Volume 20 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ikeda S, Ishizaki Y, Shimizu Y, Fujimori M, Ojima Y, Okajima M, Sugino K and Asahara T: Immunohistochemistry of cyclin D1 and β-catenin, and mutational analysis of exon 3 of β-catenin gene in parathyroid adenomas. Int J Oncol 20: 463-466, 2002
APA
Ikeda, S., Ishizaki, Y., Shimizu, Y., Fujimori, M., Ojima, Y., Okajima, M. ... Asahara, T. (2002). Immunohistochemistry of cyclin D1 and β-catenin, and mutational analysis of exon 3 of β-catenin gene in parathyroid adenomas. International Journal of Oncology, 20, 463-466. https://doi.org/10.3892/ijo.20.3.463
MLA
Ikeda, S., Ishizaki, Y., Shimizu, Y., Fujimori, M., Ojima, Y., Okajima, M., Sugino, K., Asahara, T."Immunohistochemistry of cyclin D1 and β-catenin, and mutational analysis of exon 3 of β-catenin gene in parathyroid adenomas". International Journal of Oncology 20.3 (2002): 463-466.
Chicago
Ikeda, S., Ishizaki, Y., Shimizu, Y., Fujimori, M., Ojima, Y., Okajima, M., Sugino, K., Asahara, T."Immunohistochemistry of cyclin D1 and β-catenin, and mutational analysis of exon 3 of β-catenin gene in parathyroid adenomas". International Journal of Oncology 20, no. 3 (2002): 463-466. https://doi.org/10.3892/ijo.20.3.463