Infrequent mutations of the activating transcription factor-2 gene in human lung cancer, neuroblastoma and breast cancer

  • Authors:
    • In Sook Woo
    • Takashi Kohno
    • Kaoru Inoue
    • Shunsuke Ishii
    • Jun Yokota
  • View Affiliations

  • Published online on: March 1, 2002     https://doi.org/10.3892/ijo.20.3.527
  • Pages: 527-531
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Abstract

The activating transcription factor 2 (ATF-2) gene, which encodes a transcription factor involved in multiple intracellular signal transduction pathways, is located on human chromosome 2q32, which is a common region of LOH in human lung cancer. In neuroblastoma and breast cancer, a high incidence of LOH was detected on chromosome 2q. Recently we found that breast cancer is frequently developed in heterozygous mutant mice for the ATF-2 gene. Therefore, the ATF-2 gene was considered as a candidate tumor suppressor gene on 2q. To assess the role of the ATF-2 gene as a tumor suppressor in human carcinogenesis, we examined genetic alterations of the ATF-2 gene in 9 breast cancer cell lines, 10 neuroblastoma cell lines and 46 lung cancer cell lines. For this purpose, we first determined the exon-intron structure of the ATF-2 gene in the human genome. The ATF-2 gene was composed of 14 exons and 13 introns, and the ATG start codon and the TGA stop codon were present in exons 3 and 14, respectively. Genetic variants of the ATF-2 gene were detected in 5 of the 46 (10.6%) lung cancers, but not in neuroblastomas and breast cancers. Three of the five variants detected in lung cancers were genetic polymorphisms, while the remaining two, consisting of non-synonymous and synonymous substitutions, were possibly somatic mutations. The present result indicates that the ATF-2 gene is not a major tumor suppressor gene on chromosome 2q, however, it is possible that ATF-2 alterations may be involved in the development of a small subset of lung cancers.

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March 2002
Volume 20 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Woo IS, Kohno T, Inoue K, Ishii S and Yokota J: Infrequent mutations of the activating transcription factor-2 gene in human lung cancer, neuroblastoma and breast cancer. Int J Oncol 20: 527-531, 2002
APA
Woo, I.S., Kohno, T., Inoue, K., Ishii, S., & Yokota, J. (2002). Infrequent mutations of the activating transcription factor-2 gene in human lung cancer, neuroblastoma and breast cancer. International Journal of Oncology, 20, 527-531. https://doi.org/10.3892/ijo.20.3.527
MLA
Woo, I. S., Kohno, T., Inoue, K., Ishii, S., Yokota, J."Infrequent mutations of the activating transcription factor-2 gene in human lung cancer, neuroblastoma and breast cancer". International Journal of Oncology 20.3 (2002): 527-531.
Chicago
Woo, I. S., Kohno, T., Inoue, K., Ishii, S., Yokota, J."Infrequent mutations of the activating transcription factor-2 gene in human lung cancer, neuroblastoma and breast cancer". International Journal of Oncology 20, no. 3 (2002): 527-531. https://doi.org/10.3892/ijo.20.3.527