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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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March 2002 Volume 20 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

An International Open Access Journal Devoted to General Medicine.

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March 2002 Volume 20 Issue 3

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Article

Fermented wheat germ extract induces apoptosis and downregulation of major histocompatibility complex class I proteins in tumor T and B cell lines

  • Authors:
    • Roberta Fajka-Boja
    • Mate Hidvegi
    • Yehuda Shoenfeld
    • Gabriela Ion
    • Dmytro Demydenko
    • Rita Tomoskozi-Farkas
    • Csaba Vizler
    • Andras Telekes
    • Akos Resetar
    • Eva Monostori
  • View Affiliations / Copyright

    Affiliations: Lymphocyte Signal Transduction Laboratory, Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary
  • Pages: 563-570
    |
    Published online on: March 1, 2002
       https://doi.org/10.3892/ijo.20.3.563
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Abstract

The fermented wheat germ extract (code name: MSC, trade name: Avemar), with standardized benzoquinone content has been shown to inhibit tumor propagation and metastases formation in vivo. The aim of this study was to understand the molecular and cellular mechanisms of the anti-tumor effect of MSC. Therefore, we have designed in vitro model experiments using T and B tumor lymphocytic cell lines. Tyrosine phosphorylation of intracellular proteins and elevation of the intracellular Ca2+ concentration were examined using immunoblotting with anti-phosphotyrosine antibody and cytofluorimetry by means of Ca2+ sensitive fluorescence dyes, Fluo-3AM and FuraRed-AM, respectively. Apoptosis was measured with cytofluorimetry by staining the DNA with propidium iodide and detecting the cell population. The level of the cell surface MHC class I molecules was analysed with indirect immunofluorescence on cytofluorimeter using a monoclonal antibody to the non-polymorphic region of the human MHC class I. MSC stimulated tyrosine phosphorylation of intracellular proteins and the influx of extracellular Ca2+ resulted in elevation of intracellular Ca2+ concentration. Prominent apoptosis of 20-40% was detected upon 24 h of MSC treatment of the cell lines. As a result of the MSC treatment, the amount of the cell surface MHC class I proteins was downregulated by 70-85% compared to the non-stimulated control. MSC did not induce a similar degree of apoptosis in healthy peripheral blood mononuclear cells. Inhibition of the cellular tyrosine phosphatase activity or Ca2+ influx resulted in the opposite effect increasing or diminishing the Avemar induced apoptosis as well as the MHC class I downregulation, respectively. A benzoquinone component (2,6-dimethoxi-p-benzoquinone) in MSC induced similar apoptosis and downregulation of the MHC class I molecules in the tumor T and B cell lines to that of MSC. These results suggest that MSC acts on lymphoid tumor cells by reducing MHC class I expression and selectively promoting apoptosis of tumor cells on a tyrosine phosphorylation and Ca2+ influx dependent way. One of the components in MSC, 2,6-dimethoxi-p-benzoquinone was shown to be an important factor in MSC mediated cell response.

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Copy and paste a formatted citation
Spandidos Publications style
Fajka-Boja R, Hidvegi M, Shoenfeld Y, Ion G, Demydenko D, Tomoskozi-Farkas R, Vizler C, Telekes A, Resetar A, Monostori E, Monostori E, et al: Fermented wheat germ extract induces apoptosis and downregulation of major histocompatibility complex class I proteins in tumor T and B cell lines. Int J Oncol 20: 563-570, 2002.
APA
Fajka-Boja, R., Hidvegi, M., Shoenfeld, Y., Ion, G., Demydenko, D., Tomoskozi-Farkas, R. ... Monostori, E. (2002). Fermented wheat germ extract induces apoptosis and downregulation of major histocompatibility complex class I proteins in tumor T and B cell lines. International Journal of Oncology, 20, 563-570. https://doi.org/10.3892/ijo.20.3.563
MLA
Fajka-Boja, R., Hidvegi, M., Shoenfeld, Y., Ion, G., Demydenko, D., Tomoskozi-Farkas, R., Vizler, C., Telekes, A., Resetar, A., Monostori, E."Fermented wheat germ extract induces apoptosis and downregulation of major histocompatibility complex class I proteins in tumor T and B cell lines". International Journal of Oncology 20.3 (2002): 563-570.
Chicago
Fajka-Boja, R., Hidvegi, M., Shoenfeld, Y., Ion, G., Demydenko, D., Tomoskozi-Farkas, R., Vizler, C., Telekes, A., Resetar, A., Monostori, E."Fermented wheat germ extract induces apoptosis and downregulation of major histocompatibility complex class I proteins in tumor T and B cell lines". International Journal of Oncology 20, no. 3 (2002): 563-570. https://doi.org/10.3892/ijo.20.3.563
Copy and paste a formatted citation
x
Spandidos Publications style
Fajka-Boja R, Hidvegi M, Shoenfeld Y, Ion G, Demydenko D, Tomoskozi-Farkas R, Vizler C, Telekes A, Resetar A, Monostori E, Monostori E, et al: Fermented wheat germ extract induces apoptosis and downregulation of major histocompatibility complex class I proteins in tumor T and B cell lines. Int J Oncol 20: 563-570, 2002.
APA
Fajka-Boja, R., Hidvegi, M., Shoenfeld, Y., Ion, G., Demydenko, D., Tomoskozi-Farkas, R. ... Monostori, E. (2002). Fermented wheat germ extract induces apoptosis and downregulation of major histocompatibility complex class I proteins in tumor T and B cell lines. International Journal of Oncology, 20, 563-570. https://doi.org/10.3892/ijo.20.3.563
MLA
Fajka-Boja, R., Hidvegi, M., Shoenfeld, Y., Ion, G., Demydenko, D., Tomoskozi-Farkas, R., Vizler, C., Telekes, A., Resetar, A., Monostori, E."Fermented wheat germ extract induces apoptosis and downregulation of major histocompatibility complex class I proteins in tumor T and B cell lines". International Journal of Oncology 20.3 (2002): 563-570.
Chicago
Fajka-Boja, R., Hidvegi, M., Shoenfeld, Y., Ion, G., Demydenko, D., Tomoskozi-Farkas, R., Vizler, C., Telekes, A., Resetar, A., Monostori, E."Fermented wheat germ extract induces apoptosis and downregulation of major histocompatibility complex class I proteins in tumor T and B cell lines". International Journal of Oncology 20, no. 3 (2002): 563-570. https://doi.org/10.3892/ijo.20.3.563
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