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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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March 2002 Volume 20 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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March 2002 Volume 20 Issue 3

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Article

Molecular genetic evaluation of fluorescence diagnosis in bladder cancer

  • Authors:
    • Kerstin Junker
    • Konstanze Kania
    • Wolfgang Fiedler
    • Arndt Hartmann
    • Joerg Schubert
    • Wolfram Werner

  • View Affiliations / Copyright

    Affiliations: Department of Urology, Friedrich-Schiller-University, D-07743 Jena, Germany. kerstin.junker@med.uni-jena.de
  • Pages: 647-653
    |
    Published online on: March 1, 2002
       https://doi.org/10.3892/ijo.20.3.647
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Abstract

Fluorescence diagnosis of superficial bladder cancer using 5-aminolevulinic acid (ALA) is a highly sensitive technique (95%). However, the specificity is only 60-70% due to false-positive results after histopathological examination. We hypothesized that the biopsies in fluorescence endoscopy could represent early preneoplastic lesions not detectable by histopathology. In order to evaluate the specificity of fluorescence endoscopy at the molecular genetic level we performed comparative genomic hybridization (CGH) and investigated telomerase activity of ALA-positive tissue samples. For CGH, DNA was isolated from 5-10 frozen sections. Tumor and normal (control) DNAs were amplified by DOP-PCR and labeled with biotin-dUTP and digoxigenin-dUTP, respectively. Hybridization and detection were carried out according to standard protocols. Telomerase activity was analyzed using a non-radioactive system (TRAP-assay). In 33 out of 118 bladder cancer cases (28%) detected by conventional cystoscopy, additional suspicious areas were found using ALA. CGH revealed genetic changes in 27% of samples with non-malignant histological diagnoses. Telomerase activity was found in 59% of these samples. Tumor samples showed genetic alterations in 84% and in 69% telomerase activity occurred. The type of genetic alterations in the normal biopsies was identical to the tumors. Based on these molecular data, the portion of false-positive results obtained by fluorescence diagnosis is lower than defined by histopathology alone. Genetic alterations and activation of telomerase activity are early events of tumor development in bladder cancer occurring earlier than histological features of neoplasia. The clinical importance of fluorescence diagnosis and the possible reduction of the recurrence rate have to be shown in ongoing clinical studies.

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Copy and paste a formatted citation
Spandidos Publications style
Junker K, Kania K, Fiedler W, Hartmann A, Schubert J and Werner W: Molecular genetic evaluation of fluorescence diagnosis in bladder cancer. Int J Oncol 20: 647-653, 2002.
APA
Junker, K., Kania, K., Fiedler, W., Hartmann, A., Schubert, J., & Werner, W. (2002). Molecular genetic evaluation of fluorescence diagnosis in bladder cancer. International Journal of Oncology, 20, 647-653. https://doi.org/10.3892/ijo.20.3.647
MLA
Junker, K., Kania, K., Fiedler, W., Hartmann, A., Schubert, J., Werner, W."Molecular genetic evaluation of fluorescence diagnosis in bladder cancer". International Journal of Oncology 20.3 (2002): 647-653.
Chicago
Junker, K., Kania, K., Fiedler, W., Hartmann, A., Schubert, J., Werner, W."Molecular genetic evaluation of fluorescence diagnosis in bladder cancer". International Journal of Oncology 20, no. 3 (2002): 647-653. https://doi.org/10.3892/ijo.20.3.647
Copy and paste a formatted citation
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Spandidos Publications style
Junker K, Kania K, Fiedler W, Hartmann A, Schubert J and Werner W: Molecular genetic evaluation of fluorescence diagnosis in bladder cancer. Int J Oncol 20: 647-653, 2002.
APA
Junker, K., Kania, K., Fiedler, W., Hartmann, A., Schubert, J., & Werner, W. (2002). Molecular genetic evaluation of fluorescence diagnosis in bladder cancer. International Journal of Oncology, 20, 647-653. https://doi.org/10.3892/ijo.20.3.647
MLA
Junker, K., Kania, K., Fiedler, W., Hartmann, A., Schubert, J., Werner, W."Molecular genetic evaluation of fluorescence diagnosis in bladder cancer". International Journal of Oncology 20.3 (2002): 647-653.
Chicago
Junker, K., Kania, K., Fiedler, W., Hartmann, A., Schubert, J., Werner, W."Molecular genetic evaluation of fluorescence diagnosis in bladder cancer". International Journal of Oncology 20, no. 3 (2002): 647-653. https://doi.org/10.3892/ijo.20.3.647
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