Decrease in growth factor receptors after treatment with serine protease inhibitor ONO-3403

  • Authors:
    • T. Hiwasa
    • H. Shimada
    • T. Ochiai
    • T.-L. Liu
    • Y. Yuasa
    • M. Takiguchi
    • M. Ohkoshi
  • View Affiliations

  • Published online on: April 1, 2002     https://doi.org/10.3892/ijo.20.4.797
  • Pages: 797-802
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Abstract

FOY-305 is a synthetic serine protease inhibitor and ONO-3403 is a derivative with a higher protease-inhibitory activity. The growth-suppressive effects of ONO-3403 were more prominent in Ha-ras-transformed NIH3T3 (ras-NIH) cells than in non-transformed NIH3T3 cells. After treatment of ras-NIH cells with ONO-3403 at 100-200 μg/ml, the percentage of cells found in G1 phase decreased and, concomitantly, that in S phase increased. Molecular events caused by ONO-3403 were investigated by Western blot analysis using anti-phosphotyrosine antibody. The results showed a marked decrease in the tyrosine phosphorylation level of a 180-kDa protein after treatment with ONO-3403. This 180-kDa phosphotyrosine-containing molecule which was tentatively designated pY-p180 might be platelet-derived growth factor (PDGF) receptor since addition of PDGF to serum-starved NIH3T3 cells induced a marked tyrosine phosphorylation of the same size within 5 min. This was further confirmed by immunoprecipitation of cell extract with anti-PDGF-receptor antibody followed by Western blot analysis using anti-phosphotyrosine antibody. Treatment of T.Tn human esophageal carcinoma cells with ONO-3403 caused also decrease in pY-p180, which appeared to be epidermal growth factor receptor. Thus, ONO-3403 may induce growth suppression by down-regulation of cell surface growth factor receptors.

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April 2002
Volume 20 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Hiwasa T, Shimada H, Ochiai T, Liu T, Yuasa Y, Takiguchi M and Ohkoshi M: Decrease in growth factor receptors after treatment with serine protease inhibitor ONO-3403. Int J Oncol 20: 797-802, 2002.
APA
Hiwasa, T., Shimada, H., Ochiai, T., Liu, T., Yuasa, Y., Takiguchi, M., & Ohkoshi, M. (2002). Decrease in growth factor receptors after treatment with serine protease inhibitor ONO-3403. International Journal of Oncology, 20, 797-802. https://doi.org/10.3892/ijo.20.4.797
MLA
Hiwasa, T., Shimada, H., Ochiai, T., Liu, T., Yuasa, Y., Takiguchi, M., Ohkoshi, M."Decrease in growth factor receptors after treatment with serine protease inhibitor ONO-3403". International Journal of Oncology 20.4 (2002): 797-802.
Chicago
Hiwasa, T., Shimada, H., Ochiai, T., Liu, T., Yuasa, Y., Takiguchi, M., Ohkoshi, M."Decrease in growth factor receptors after treatment with serine protease inhibitor ONO-3403". International Journal of Oncology 20, no. 4 (2002): 797-802. https://doi.org/10.3892/ijo.20.4.797