UDP-GlcNAc2-epimerase regulates cell surface sialylation and cell adhesion to extracellular matrix in Burkitt's lymphoma

  • Authors:
    • Osamu Suzuki
    • Yoshihiro Nozawa
    • Takanori Kawaguchi
    • Masafumi Abe
  • View Affiliations

  • Published online on: May 1, 2002     https://doi.org/10.3892/ijo.20.5.1005
  • Pages: 1005-1011
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Abstract

Alteration of cell surface glycoconjugates plays a crucial role in many biological phenomenon, including invasion and metastasis. In the present study, we investigated the relationship between cell surface sialylation and the adhesive properties of two clones (3G3 and 3D2) of a human Burkitt's lymphoma cell line, HBL-8 to extracellular matrix. By flow cytometric analysis we found that the cell surface of 3G3 clone was highly sialylated and that of the 3D2 clone were hyposialylated. Moreover, cell surface sialic acid content was significantly greater in the 3G3 clone than in the 3D2 clone. In vitro adhesion assays showed that cell surface sialylation in the 3G3 clone cells might reduce their attachment to collagen type IV and fibronectin, compared to 3D2 clone. Sialic acid metabolic complementation assays using several precursors of sialic acid suggested that hyposialylation in the 3D2 clone resulted from low activities of uridine diphosphate-N-acetylglucosamine-2-epimerase (UDP-GlcNAc2-epimerase) which is a key enzyme in sialic acid biosynthesis. From RT-PCR analysis the expression of UDP-GlcNAc2-epimerase mRNA was found to be correlated with sialic acid content in the two clones of HBL-8. Therefore, expression of UDP-GlcNAc2-epimerase mRNA may regulate the expression of sialoglycoconjugates which affect the adhesion of lymphoma cells to collagen type IV and fibronectin.

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May 2002
Volume 20 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Suzuki O, Nozawa Y, Kawaguchi T and Abe M: UDP-GlcNAc2-epimerase regulates cell surface sialylation and cell adhesion to extracellular matrix in Burkitt's lymphoma. Int J Oncol 20: 1005-1011, 2002
APA
Suzuki, O., Nozawa, Y., Kawaguchi, T., & Abe, M. (2002). UDP-GlcNAc2-epimerase regulates cell surface sialylation and cell adhesion to extracellular matrix in Burkitt's lymphoma. International Journal of Oncology, 20, 1005-1011. https://doi.org/10.3892/ijo.20.5.1005
MLA
Suzuki, O., Nozawa, Y., Kawaguchi, T., Abe, M."UDP-GlcNAc2-epimerase regulates cell surface sialylation and cell adhesion to extracellular matrix in Burkitt's lymphoma". International Journal of Oncology 20.5 (2002): 1005-1011.
Chicago
Suzuki, O., Nozawa, Y., Kawaguchi, T., Abe, M."UDP-GlcNAc2-epimerase regulates cell surface sialylation and cell adhesion to extracellular matrix in Burkitt's lymphoma". International Journal of Oncology 20, no. 5 (2002): 1005-1011. https://doi.org/10.3892/ijo.20.5.1005