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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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February 2011 Volume 38 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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February 2011 Volume 38 Issue 2

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Article

Reverse-phase protein microarrays (RPPA) as a diagnostic and therapeutic guide in multidrug resistant leukemia

  • Authors:
    • Tullia Maraldi
    • Jessika Bertacchini
    • Marta Benincasa
    • Marianna Guida
    • Anto De Pol
    • Lance A. Liotta
    • Emanuel Petricoin
    • Lucio Cocco
    • Sandra Marmiroli

  • View Affiliations / Copyright

    Affiliations: Department of Anatomy and Histology and CIPro Proteomics Centre, University of Modena and Reggio Emilia, I-41100 Modena, Italy, Department of Anatomy and Histology, University of Modena and Reggio Emilia, Via Del Pozzo 71, I-41100 Modena, Italy
  • Pages: 427-435
    |
    Published online on: December 2, 2010
       https://doi.org/10.3892/ijo.2010.850
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Abstract

Reverse-phase microarray assays using phospho-specific antibodies (RPPA) can directly measure levels of phosphorylated protein isoforms. In the current study, lysates from parental and multidrug resistant (MDR) CEM leukemia cells were spotted onto reverse-phase protein microarrays and probed with a panel of phospho-antibodies to ERK, PCK and Akt pathways. In particular, the Akt pathway is considered to play significant roles in leukemia and Akt inhibitor therapy has been proposed as a potential tool in the treatment of this disease. The RPPA data prompted us to investigate deeper this pathway. Here, we found that whereas total Akt1 protein level is higher in parental CEM cells, the activated isoform content, p-Akt1, increases in doxorubicin-selected CEM cells (MDR-CEM). This was backed up by Western blot analysis, confirming that Akt1 activity/phosphorylation may be up-regulated in MDR-CEM cells. Further exploration of inhibitory therapy in this system was evaluated. The TNF-related apoptosis-inducing ligand, TRAIL, has been shown to selectively kill tumor cells. Herein, we describe that in MDR-CEM cells TRAIL responsiveness correlates with a reduced expression of endogenous Akt1, suggesting that the MDR phenotype associated to P-gp sensitizes cells to TRAIL therapy.

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Copy and paste a formatted citation
Spandidos Publications style
Maraldi T, Bertacchini J, Benincasa M, Guida M, De Pol A, Liotta LA, Petricoin E, Cocco L and Marmiroli S: Reverse-phase protein microarrays (RPPA) as a diagnostic and therapeutic guide in multidrug resistant leukemia. Int J Oncol 38: 427-435, 2011.
APA
Maraldi, T., Bertacchini, J., Benincasa, M., Guida, M., De Pol, A., Liotta, L.A. ... Marmiroli, S. (2011). Reverse-phase protein microarrays (RPPA) as a diagnostic and therapeutic guide in multidrug resistant leukemia. International Journal of Oncology, 38, 427-435. https://doi.org/10.3892/ijo.2010.850
MLA
Maraldi, T., Bertacchini, J., Benincasa, M., Guida, M., De Pol, A., Liotta, L. A., Petricoin , E., Cocco, L., Marmiroli, S."Reverse-phase protein microarrays (RPPA) as a diagnostic and therapeutic guide in multidrug resistant leukemia". International Journal of Oncology 38.2 (2011): 427-435.
Chicago
Maraldi, T., Bertacchini, J., Benincasa, M., Guida, M., De Pol, A., Liotta, L. A., Petricoin , E., Cocco, L., Marmiroli, S."Reverse-phase protein microarrays (RPPA) as a diagnostic and therapeutic guide in multidrug resistant leukemia". International Journal of Oncology 38, no. 2 (2011): 427-435. https://doi.org/10.3892/ijo.2010.850
Copy and paste a formatted citation
x
Spandidos Publications style
Maraldi T, Bertacchini J, Benincasa M, Guida M, De Pol A, Liotta LA, Petricoin E, Cocco L and Marmiroli S: Reverse-phase protein microarrays (RPPA) as a diagnostic and therapeutic guide in multidrug resistant leukemia. Int J Oncol 38: 427-435, 2011.
APA
Maraldi, T., Bertacchini, J., Benincasa, M., Guida, M., De Pol, A., Liotta, L.A. ... Marmiroli, S. (2011). Reverse-phase protein microarrays (RPPA) as a diagnostic and therapeutic guide in multidrug resistant leukemia. International Journal of Oncology, 38, 427-435. https://doi.org/10.3892/ijo.2010.850
MLA
Maraldi, T., Bertacchini, J., Benincasa, M., Guida, M., De Pol, A., Liotta, L. A., Petricoin , E., Cocco, L., Marmiroli, S."Reverse-phase protein microarrays (RPPA) as a diagnostic and therapeutic guide in multidrug resistant leukemia". International Journal of Oncology 38.2 (2011): 427-435.
Chicago
Maraldi, T., Bertacchini, J., Benincasa, M., Guida, M., De Pol, A., Liotta, L. A., Petricoin , E., Cocco, L., Marmiroli, S."Reverse-phase protein microarrays (RPPA) as a diagnostic and therapeutic guide in multidrug resistant leukemia". International Journal of Oncology 38, no. 2 (2011): 427-435. https://doi.org/10.3892/ijo.2010.850
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