Expression of CD24 in cholangiocarcinoma cells is associated with disease progression and reduced patient survival

Keeratichamroen,Siriporn; Leelawat,Kawin; Thongtawee,Taweesak; Narong,Siriluck; Aegem,Umaad; Tujinda,Supathip; Praditphol,Niphon; Tohtong,Rutaiwan

doi:10.3892/ijo.2011.1088

October 2011 Volume 39 Issue 4

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October 2011 Volume 39 Issue 4

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Article

Expression of CD24 in cholangiocarcinoma cells is associated with disease progression and reduced patient survival

Authors:
- Siriporn Keeratichamroen
- Kawin Leelawat
- Taweesak Thongtawee
- Siriluck Narong
- Umaad Aegem
- Supathip Tujinda
- Niphon Praditphol
- Rutaiwan Tohtong
View Affiliations / Copyright

Affiliations: Department of Molecular Medicine, Faculty of Science, Mahidol University, Bangkok 10400, Thailand, Department of Surgery, Rajavithi Hospital, Bangkok 10400, Thailand
Pages: 873-881
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Published online on: June 17, 2011

https://doi.org/10.3892/ijo.2011.1088
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Abstract

Cholangiocarcinoma is frequently found to invade local tissues and metastasize to distal organs. We investigated the expression of CD24 in cholangiocarcinoma samples and its prognostic significance. In addition, the cellular function of CD24 was studied in the RMCCA1 cholangiocarcinoma cell line. High CD24 expression significantly correlated with lymph node metastasis and positive surgical margins in cholangiocarcinoma patients. Univariate and multivariate analyses further demonstrated that CD24 expression was significantly associated with the overall survival of these patients (p=0.007 and p=0.040, respectively). For in vitro studies, the magnetic-activated cell sorting (MACS) system was used to isolate CD24+ and CD24- cell populations from RMCCA1 cells. CD24+ RMCCA1 cells had increased chemoresistance, adhesion (p=0.004), motility (p<0.001), migration (p<0.001) and invasion (p<0.001) capabilities when compared to CD24- cells. The matrix metalloproteinase (MMP)-7 was significantly elevated in CD24+ RMCCA1 cells (p=0.01). We found that inhibition of CD24 using siRNA silencing significantly decreased the invasive capacity of RMCCA1 cells. Both clinical and in vitro studies suggest that expression of CD24 is associated with cholangiocarcinoma disease progression. CD24 may thus serve as a new target for directed molecular therapy of cholangiocarcinoma.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Expression of CD24 in cholangiocarcinoma cells is associated with disease progression and reduced patient survival

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Abstract

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