Lentiviral short hairpin RNA screen of human kinases and phosphatases to identify potential biomarkers in oral squamous cancer cells

  • Authors:
    • Ming-Han Yeh
    • Tzung-Chieh Tsai
    • Han-Peng Kuo
    • Nai-Wen Chang
    • Miao-Rong Lee
    • Jing-Gung Chung
    • Ming-Hsui Tsai
    • Jah-Yao Liu
    • Ming-Ching Kao
  • View Affiliations

  • Published online on: June 24, 2011     https://doi.org/10.3892/ijo.2011.1100
  • Pages: 1221-1231
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Abstract

Oral carcinoma is a serious public health problem and the leading cause of head and neck cancer mortality worldwide. Moreover, oral cancer patients often present symptoms at a late stage and show a high recurrence rate after treatment. Therefore, there is an urgent need to identify novel biomarkers for early diagnosis or clinical oral cancer therapy. In this study, we employed a subset of lentiviral short hairpin RNAs targeted against various kinases and phosphatases, designed by The RNAi Consortium, to screen systemically and in a high-throughput manner for potential growth regulators of oral cancer cells. The screen revealed a total of 50 candidate genes, for which more than 90% of growth inhibition in human oral squamous cancer HSC-3 cells was obtained. Furthermore, bioinformatic analysis of these candidate genes identified transforming growth factor-β receptor type II- and fms-related tyrosine kinase 3-related molecular pathways that are involved in NF-κB-mediated growth of HSC-3 cells. These candidate genes may be potential biomarkers for early diagnosis of oral cancer. In addition, these candidate genes represent potential targets for anticancer drug design helping to develop a personalized treatment to combat oral cancer.

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November 2011
Volume 39 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Yeh, M., Tsai, T., Kuo, H., Chang, N., Lee, M., Chung, J. ... Kao, M. (2011). Lentiviral short hairpin RNA screen of human kinases and phosphatases to identify potential biomarkers in oral squamous cancer cells. International Journal of Oncology, 39, 1221-1231. https://doi.org/10.3892/ijo.2011.1100
MLA
Yeh, M., Tsai, T., Kuo, H., Chang, N., Lee, M., Chung, J., Tsai, M., Liu, J., Kao, M."Lentiviral short hairpin RNA screen of human kinases and phosphatases to identify potential biomarkers in oral squamous cancer cells". International Journal of Oncology 39.5 (2011): 1221-1231.
Chicago
Yeh, M., Tsai, T., Kuo, H., Chang, N., Lee, M., Chung, J., Tsai, M., Liu, J., Kao, M."Lentiviral short hairpin RNA screen of human kinases and phosphatases to identify potential biomarkers in oral squamous cancer cells". International Journal of Oncology 39, no. 5 (2011): 1221-1231. https://doi.org/10.3892/ijo.2011.1100