Lentiviral short hairpin RNA screen of human kinases and phosphatases to identify potential biomarkers in oral squamous cancer cells
- Ming-Han Yeh
- Tzung-Chieh Tsai
- Han-Peng Kuo
- Nai-Wen Chang
- Miao-Rong Lee
- Jing-Gung Chung
- Ming-Hsui Tsai
- Jah-Yao Liu
- Ming-Ching Kao
Affiliations: Graduate Institute of Life Sciences, National Defense Medical Center, No. 161, Section 6, Min-Chuan East Road, Taipei, Taiwan 11490, R.O.C., Department of Obstetrics and Gynecology, Tri-Service General Hospital, No. 325, Section 2, Chenggong Road, Taipei, Taiwan 11490, R.O.C., Department of Biological Science and Technology, College of Life Sciences, China Medical University, 91 Hsueh-Shih Road, Taichung, Taiwan 40402, R.O.C.
- Published online on: June 24, 2011 https://doi.org/10.3892/ijo.2011.1100
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Oral carcinoma is a serious public health problem and the leading cause of head and neck cancer mortality worldwide. Moreover, oral cancer patients often present symptoms at a late stage and show a high recurrence rate after treatment. Therefore, there is an urgent need to identify novel biomarkers for early diagnosis or clinical oral cancer therapy. In this study, we employed a subset of lentiviral short hairpin RNAs targeted against various kinases and phosphatases, designed by The RNAi Consortium, to screen systemically and in a high-throughput manner for potential growth regulators of oral cancer cells. The screen revealed a total of 50 candidate genes, for which more than 90% of growth inhibition in human oral squamous cancer HSC-3 cells was obtained. Furthermore, bioinformatic analysis of these candidate genes identified transforming growth factor-β receptor type II- and fms-related tyrosine kinase 3-related molecular pathways that are involved in NF-κB-mediated growth of HSC-3 cells. These candidate genes may be potential biomarkers for early diagnosis of oral cancer. In addition, these candidate genes represent potential targets for anticancer drug design helping to develop a personalized treatment to combat oral cancer.