Open Access

Evaluation of effects caused by differentially spliced Ets-1 transcripts in fibroblasts

  • Authors:
    • Jens Claus Hahne
    • Tanja Fuchs
    • Alexandra Florin
    • Dylan Edwards
    • Albin Pourtier
    • Fabrice Soncin
    • Nicolas Wernert
  • View Affiliations

  • Published online on: August 9, 2011     https://doi.org/10.3892/ijo.2011.1152
  • Pages: 1073-1082
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Abstract

The transcription factor Ets-1 is known to be involved in a broad variety of cellular functions such as cell proliferation, migration, invasion, apoptosis and angiogenesis. In nearly all these reports, the full-length Ets-1 (p51) is commonly considered to be the active form and the role of the Ets-1ΔVII splice variant (p42) has not been addressed. Therefore, we studied the functional effects of p42 Ets-1 in comparison to p51 Ets-1 expression in a well-characterized mouse fibroblast cell line. Furthermore, the specific role of Ets-1 was evaluated using mouse fibroblasts with a reduced Ets-1 expression caused by RNAi and compared to fibroblasts with a binding inhibition of the whole ETS transcription factor family by stably overexpressing the ETS DNA binding domain as transdominant-negative mutant. Our results demonstrate that p42 Ets-1 has quite different functions and target genes compared to p51 Ets-1 (e.g. TIMP-4, MMP-3, MMP-9, MMP-13). In some cases (e.g. in cytokine expression) p42 Ets-1 is a functional transcription factor which acts in the same manner as a transdominant-negative approach.

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November 2011
Volume 39 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Hahne, J.C., Fuchs, T., Florin, A., Edwards, D., Pourtier, A., Soncin, F., & Wernert, N. (2011). Evaluation of effects caused by differentially spliced Ets-1 transcripts in fibroblasts. International Journal of Oncology, 39, 1073-1082. https://doi.org/10.3892/ijo.2011.1152
MLA
Hahne, J. C., Fuchs, T., Florin, A., Edwards, D., Pourtier, A., Soncin, F., Wernert, N."Evaluation of effects caused by differentially spliced Ets-1 transcripts in fibroblasts". International Journal of Oncology 39.5 (2011): 1073-1082.
Chicago
Hahne, J. C., Fuchs, T., Florin, A., Edwards, D., Pourtier, A., Soncin, F., Wernert, N."Evaluation of effects caused by differentially spliced Ets-1 transcripts in fibroblasts". International Journal of Oncology 39, no. 5 (2011): 1073-1082. https://doi.org/10.3892/ijo.2011.1152